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验证数据展示

  • IF Staining of HeLa using CL488-81011

    IF Staining of HeLa using CL488-81011

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 OXCT1 antibody (CL488-81011, Clone: 4K12 ) at dilution of 1:200.

  • FC experiment of MCF-7 using CL488-81011

    FC experiment of MCF-7 using CL488-81011

    1X10^6 MCF-7 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human OXCT1 (CL488-81011, Clone:4K12) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of MCF-7 using CL488-81011

    IF Staining of MCF-7 using CL488-81011

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite® Plus 488 OXCT1 antibody (CL488-81011, Clone: 4K12 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated OXCT1 Recombinant antibody

OXCT1 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra)
Cat No. CL488-81011

产品说明书

CloneNo. 4K12

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

SCOT, OXCT, EC:2.8.3.5, 4K12, 3-oxoacid CoA-transferase 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells, MCF-7 cells
Positive FC (Intra) detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy OXCT1 Ready-To-Use IHC Kit. OXCT1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 81011-1-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-81011 targets OXCT1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 OXCT1 fusion protein Ag2818 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 3-oxoacid CoA transferase 1
别名 SCOT, OXCT, EC:2.8.3.5, 4K12, 3-oxoacid CoA-transferase 1
计算分子量 520 aa, 56 kDa
观测分子量56 kDa
GenBank蛋白编号BC009001
基因名称 OXCT1
Gene ID (NCBI) 5019
RRIDAB_3084497
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP55809
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

3-oxoacid-CoA transferase 1 (OXCT1), encoded by nuclear gene, is a mitochondrial CoA transferase required for ketone body degradation. It catalyzes the transfer of CoA from succinyl-CoA to acetoacetate, generating acetoacetyl-CoA. OXCT1 is expressed in brain, heart, and skeletal muscle, but not in liver. This antibody specifically recognizes endogenous OXCT1. (21209089)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 OXCT1 antibody CL488-81011Download protocol
FC protocol for CL Plus 488 OXCT1 antibody CL488-81011Download protocol
Standard Protocols
Click here to view our Standard Protocols