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CoraLite® Plus 488-conjugated OXCT1 Recombinant antibody

OXCT1 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

4K12

Cat no : CL488-81011

Print datasheet

Synonyms

SCOT, OXCT, EC:2.8.3.5, 4K12, 3-oxoacid CoA-transferase 1

验证数据展示

  • IF Staining of HeLa using CL488-81011

    IF Staining of HeLa using CL488-81011

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 OXCT1 antibody (CL488-81011, Clone: 4K12 ) at dilution of 1:200.

  • FC experiment of MCF-7 using CL488-81011

    FC experiment of MCF-7 using CL488-81011

    1X10^6 MCF-7 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human OXCT1 (CL488-81011, Clone:4K12) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of MCF-7 using CL488-81011

    IF Staining of MCF-7 using CL488-81011

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite® Plus 488 OXCT1 antibody (CL488-81011, Clone: 4K12 ) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inHeLa cells, MCF-7 cells
Positive FC (Intra) detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy OXCT1 Ready-To-Use IHC Kit. OXCT1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 81011-1-PBS

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-81011 targets OXCT1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen OXCT1 fusion protein Ag2818 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name 3-oxoacid CoA transferase 1
Synonyms SCOT, OXCT, EC:2.8.3.5, 4K12, 3-oxoacid CoA-transferase 1
Calculated Molecular Weight 520 aa, 56 kDa
Observed Molecular Weight56 kDa
GenBank Accession NumberBC009001
Gene Symbol OXCT1
Gene ID (NCBI) 5019
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP55809
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

3-oxoacid-CoA transferase 1 (OXCT1), encoded by nuclear gene, is a mitochondrial CoA transferase required for ketone body degradation. It catalyzes the transfer of CoA from succinyl-CoA to acetoacetate, generating acetoacetyl-CoA. OXCT1 is expressed in brain, heart, and skeletal muscle, but not in liver. This antibody specifically recognizes endogenous OXCT1. (21209089)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 OXCT1 antibody CL488-81011Download protocol
FC protocol for CL Plus 488 OXCT1 antibody CL488-81011Download protocol
Standard Protocols
Click here to view our Standard Protocols