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验证数据展示

  • WB analysis using 27260-1-AP

    WB analysis using 27260-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27260-1-AP (Occludin antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours.

  • WB analysis using 27260-1-AP

    WB analysis using 27260-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27260-1-AP (Occludin antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours.

  • IP experiment of HUVEC using 27260-1-AP

    IP experiment of HUVEC using 27260-1-AP

    IP result of anti-Occludin (IP:27260-1-AP, 4ug; Detection:27260-1-AP 1:2000) with HUVEC cells lysate 840 ug.

  • IHC staining of mouse colon using 27260-1-AP

    IHC staining of mouse colon using 27260-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 27260-1-AP (Occludin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse colon using 27260-1-AP

    IHC staining of mouse colon using 27260-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 27260-1-AP (Occludin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon using 27260-1-AP

    IHC staining of human colon using 27260-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27260-1-AP (Occludin antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse brain using 27260-1-AP

    IF Staining of mouse brain using 27260-1-AP

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using Occludin antibody (27260-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IF Staining of Caco-2 using 27260-1-AP

    IF Staining of Caco-2 using 27260-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using Occludin antibody (27260-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

  • FC experiment of MCF-7 using 27260-1-AP

    FC experiment of MCF-7 using 27260-1-AP

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug Occludin Polyclonal antibody (27260-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).

  • IHC staining of human colon using 27260-1-AP

    IHC staining of human colon using 27260-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27260-1-AP (Occludin antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat colon using 27260-1-AP

    IHC staining of rat colon using 27260-1-AP

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 27260-1-AP (Occludin antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse colon using 27260-1-AP

    IF Staining of mouse colon using 27260-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse colon tissue using Occludin antibody (27260-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Occludin Polyclonal antibody

Occludin Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), IP, ELISA
Cat No. 27260-1-AP

产品说明书

引用文献(1340)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (6)

应用

WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), IP, ELISA and More (1)

别名

OCLN

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHUVEC cells, A431 cells, mouse kidney tissue, Caco-2 cells, mouse brain tissue, mouse colon tissue, rat colon tissue, rat brain tissue
Positive IP detected inHUVEC cells
Positive IHC detected inmouse colon tissue, human colon tissue, rat colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse colon tissue
Positive IF-Fro detected inmouse brain tissue
Positive IF/ICC detected inCaco-2 cells, MCF-7 cells, hTERT-RPE1 cells
Positive FC (Intra) detected inMCF-7 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:400-1:2000
Immunofluorescence (IF)-FROIF-FRO : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27260-1-AP targets Occludin in WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat, pig, rabbit, chicken, sheep, goat, duck
免疫原

CatNo: Ag26173

Product name: Recombinant human Occludin protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 360-522 aa of BC029886

Sequence: VDDFRQPRYSSGGNFETPSKRAPAKGRAGRSKRTEQDHYETDYTTGGESCDELEEDWIREYPPITSDQQRQLYKRNFDTGLQEYKSLQSELDEINKELSRLDKELDDYREESEEYMAAADEYNRLKQVKGSADYKSKKNHCKQLKSKLSHIKKMVGDYDRQKT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 occludin
别名 OCLN
计算分子量 522 aa, 59 kDa
观测分子量 59 kDa, 53 kDa, 32 kDa, 23-25 kDa
GenBank蛋白编号BC029886
基因名称 Occludin
Gene ID (NCBI) 4950
RRIDAB_2880820
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ16625
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Occludin is an integral membrane protein located at the tight junction. It is a tetraspanin protein with four transmembrane domains, intracellular N and C termini, and two extracellular loops. Occludin plays a role in the formation and regulation of the tight junction paracellular permeability barrier. Occludin can exist in different isoforms, owing to modifications at the posttranscriptional and posttranslational levels, the monomeric occludin migrates as 53-65 kDa on SDS-PAGE (PMID: 22083955; 19457074).

实验方案

Product Specific Protocols
FC protocol for Occludin antibody 27260-1-APDownload protocol
IF protocol for Occludin antibody 27260-1-APDownload protocol
IHC protocol for Occludin antibody 27260-1-APDownload protocol
IP protocol for Occludin antibody 27260-1-APDownload protocol
WB protocol for Occludin antibody 27260-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

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