CoraLite® Plus 488-conjugated Occludin Polyclonal antibody

Occludin Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-27260

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

IF/ICC, FC (Intra) and More (1)

OCLN

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive IF/ICC detected inMCF-7 cells, Caco-2 cells
Positive FC (Intra) detected inMCF-7 cells
For other applications, we recommend the unconjugated version of this antibody, 27260-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 2 publications below

产品信息

CL488-27260 targets Occludin in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
文献引用应用IF
经测试反应性 human
文献引用反应性human, mouse
免疫原 Occludin fusion protein Ag26173 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 occludin
别名 OCLN
计算分子量 522 aa, 59 kDa
GenBank蛋白编号BC029886
基因名称 Occludin
Gene ID (NCBI) 4950
RRIDAB_2919212
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ16625
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Occludin is an integral membrane protein located at the tight junction. It is a tetraspanin protein with four transmembrane domains, intracellular N and C termini and two extracellular loops. Occludin plays a role in the formation and regulation of the tight junction paracellular permeability barrier. Occludin can exist in different isoforms, owing to modifications at the posttranscriptional and posttranslational levels, the monomeric occludin migrates as 53-65 kDa on SDS-PAGE (PMID: 22083955; 19457074).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Occludin antibody CL488-27260Download protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

J Neurodev Disord

Rescue of impaired blood-brain barrier in tuberous sclerosis complex patient derived neurovascular unit

Authors - Jacquelyn A Brown
mouseIF

Cell

The choroid plexus synergizes with immune cells during neuroinflammation

Authors - Huixin Xu
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