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CoraLite®594-conjugated Osteocalcin Polyclonal antibody

Osteocalcin Polyclonal Antibody for FC (Intra), IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

Cat No : CL594-23418

Print datasheet

Synonyms

BGLAP, BGP, Bone Gla protein, OC, Osteocalcin, PMF1

验证数据展示

  • IF Staining of NIH/3T3 using CL594-23418

    IF Staining of NIH/3T3 using CL594-23418

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using CoraLite®594 Osteocalcin antibody (CL594-23418) at dilution of 1:400.

  • FC experiment of NIH/3T3 using CL594-23418

    FC experiment of NIH/3T3 using CL594-23418

    1X10^6 NIH/3T3 cells were intracellularly stained with 0.5 ug CoraLite®594 Anti-Human Osteocalcin (CL594-23418) (red), or 0.5 ug CoraLite®594-conjugated Rabbit IgG control Rabbit PolyAb (CL594-30000, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF detected inNIH/3T3 cells
Positive FC detected inNIH/3T3 cells
For other applications, we recommend the unconjugated version of this antibody, 23418-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-23418 targets Osteocalcin in IF, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Applications FC (Intra), IF/ICC
Tested Reactivity human, mouse
Immunogen Osteocalcin fusion protein Ag20065 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name bone gamma-carboxyglutamate (gla) protein
Synonyms BGLAP, BGP, Bone Gla protein, OC, Osteocalcin, PMF1
Calculated Molecular Weight 100 aa, 11 kDa
GenBank Accession NumberBC113432
Gene Symbol BGLAP
Gene ID (NCBI) 632
RRIDAB_2919870
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP02818
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL594 Osteocalcin antibody CL594-23418Download protocol
Standard Protocols
Click here to view our Standard Protocols