验证数据展示
经过测试的应用
| Positive WB detected in | RAW 264.7 cells |
| Positive IF-P detected in | mouse stomach tissue |
| Positive IF/ICC detected in | LPS and Brefeldin A treated RAW 264.7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
83413-6-RR targets Osteopontin in WB, IF/ICC, IF-P, ELISA applications and shows reactivity with mouse samples.
| 经测试应用 | WB, IF/ICC, IF-P, ELISA Application Description |
| 经测试反应性 | mouse |
| 免疫原 | Recombinant protein 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | secreted phosphoprotein 1 |
| 别名 | Spp1, Opn, 240250A1 |
| 计算分子量 | 32kd |
| 观测分子量 | 60 kDa |
| GenBank蛋白编号 | NM_001204233.1 |
| 基因名称 | Osteopontin |
| Gene ID (NCBI) | 20750 |
| RRID | AB_3671061 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | Q547B5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Osteopontin (OPN) is also named as 2AR, minopontin, bone sialoprotein 1, Spp1, Eta-1 and Op, and belongs to the osteopontin family. Osteopontin is major non-collagenous bone protein that binds tightly to hydroxyapatite. It is a highly phosphorylated glycophosphoprotein having acidic characteristics and rich in aspartic acid (PMID: 30003880). And it acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity (PMID:2351930, PMID:10657301). OPN, a multifunctional protein, has important functions on cardiovascular diseases, cancer, diabetes and kidney stone diseases and in the process of inflammation, biomineralization, cell viability and wound healing (PMID: 30003880). Osteopontin (OPN) is a pleiotropic glycoprotein expressed in various cell types in animals and in humans, including bone, immune, smooth muscle, epithelial and endothelial cells. Moreover, OPN is found in kidneys (in the thick ascending limbs of the loop of Henle and in distal nephrons) and urine (PMID:30456594).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Osteopontin antibody 83413-6-RR | Download protocol |
| IF protocol for Osteopontin antibody 83413-6-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |