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Mouse Osteopontin Recombinant antibody, PBS Only

Osteopontin Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240250A1

Cat no : 83413-6-PBS

Print datasheet

Synonyms

Spp1, Opn, 240250A1

验证数据展示

  • WB analysis of RAW 264.7 using 83413-6-RR (same clone as 83413-6-PBS)

    WB analysis of RAW 264.7 using 83413-6-RR (same clone as 83413-6-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 83413-6-RR (Osteopontin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83413-6-PBS in a different storage buffer formulation.

  • IF Staining of RAW 264.7 using 83413-6-RR (same clone as 83413-6-PBS)

    IF Staining of RAW 264.7 using 83413-6-RR (same clone as 83413-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed LPS and Brefeldin A treated RAW 264.7 cells using Osteopontin antibody (83413-6-RR, Clone: 240250A1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83413-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83413-6-RR

    Affinity and Kinetic Characterization of 83413-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83413-6-RR against Mouse Osteopontin were performed. The affinity constant is 2.19 nM.

产品信息

83413-6-PBS targets Osteopontin in WB, IF/ICC, ELISA applications and shows reactivity with mouse samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity mouse
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name secreted phosphoprotein 1
Synonyms Spp1, Opn, 240250A1
Calculated Molecular Weight32kd
GenBank Accession NumberNM_001204233.1
Gene Symbol Osteopontin
Gene ID (NCBI) 20750
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ547B5
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C