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验证数据展示

  • WB analysis using 84295-3-RR

    WB analysis using 84295-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84295-3-RR (P glycoprotein antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

P glycoprotein Recombinant antibody

P glycoprotein Recombinant Antibody for WB, ELISA
Cat No. 84295-3-RR

Print datasheet

CloneNo. 241512A1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

ABCB1, 241512A1, EC:7.6.2.2, EC:7.6.2.1, ATP-dependent translocase ABCB1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inPC-3 cells, HuH-7 cells, HepG2 cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy ABCB1 Ready-To-Use IHC Kit. ABCB1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84295-3-RR targets P glycoprotein in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ATP-binding cassette, sub-family B (MDR/TAP), member 1
Synonyms ABCB1, 241512A1, EC:7.6.2.2, EC:7.6.2.1, ATP-dependent translocase ABCB1
Calculated Molecular Weight 1280 aa, 142 kDa
Observed Molecular Weight 130-150 kDa
GenBank Accession NumberBC130424
Gene Symbol P glycoprotein
Gene ID (NCBI) 5243
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP08183
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ABCB1 (also known as P-gp/ P-glycoprotein, MDR1/multidrug-resistance 1) is a plasma membrane protein first discovered in multidrug resistant tumor cells. ABCB1 can extrude a large number of medically relevant compounds from cells and causes drug resistance. ABCB1 is expressed in a variety of human cancers as well as normal tissues including brain, and placenta. Overexpression of ABCB1 correlates with a negative prognosis in several types of cancer.

实验方案

Product Specific Protocols
WB protocol for P glycoprotein antibody 84295-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols