P16-INK4A Monoclonal antibody

P16-INK4A Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 60626-1-Ig

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CloneNo. 1C9D3

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

p16, p16 INK4, P16 INK4A, p16-INK4, p16INK4A

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated

Size: 
50 μL
100 μL
150 μL

¥2200/ 100 μL

浓度: 1000 ug/ml

库存:现货


经过测试的应用

Positive WB detected inSiHa cells, HeLa cells, HEK-293 cells, TF-1 cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CDKN2A/P16INK4A Ready-To-Use IHC Kit. CDKN2A/P16INK4A primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60626-1-Ig targets P16-INK4A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen P16-INK4A fusion protein Ag1328 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name cyclin-dependent kinase inhibitor 2A
Synonyms p16, p16 INK4, P16 INK4A, p16-INK4, p16INK4A
Calculated Molecular Weight 16 kDa
Observed Molecular Weight 16 kDa
GenBank Accession NumberBC021998
Gene Symbol CDKN2A
Gene ID (NCBI) 1029
RRIDAB_3670270
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP42771
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

P16-INK4A is also named as CDKN2A, MLM, Tumor suppressor ARF, Alternative reading frame. The tumor suppressor protein p16Ink4a (encoded from the CDKN2A locus) is often transcriptionally activated in cells undergoing senescence and is one of the main regulators of this program, and it is upregulated in multiple tissues during aging (PMID:17055429). p16-Ink4a is the principal member of the Ink4 family of CDK inhibitors. p16-Ink4a contributes to the regulation of cell cycle progression by inhibiting the S phase. p16Ink4a binds to CDK4/6, inhibiting cyclin D-CDK4/6 complex formation and CDK4/6-mediated phosphorylation of Rb family members. Expression of p16-Ink4a maintains the Rb family members in a hypophosphorylated state, which promotes binding to E2F1 and leads to G1 cell cycle arrest (PMID: 21297668).

实验方案

Product Specific Protocols
WB protocol for P16-INK4A antibody 60626-1-IgDownload protocol
IHC protocol for P16-INK4A antibody 60626-1-IgDownload protocol
IF protocol for P16-INK4A antibody 60626-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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