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验证数据展示

  • IF Staining of HeLa using 60626-1-Ig (same clone as 60626-1-PBS)

    IF Staining of HeLa using 60626-1-Ig (same clone as 60626-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using P16-INK4A antibody (60626-1-Ig, Clone: 1C9D3 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 60626-1-PBS in a different storage buffer formulation.

  • WB analysis using 60626-1-Ig (same clone as 60626-1-PBS)

    WB analysis using 60626-1-Ig (same clone as 60626-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60626-1-Ig (P16-INK4A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60626-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 60626-1-Ig (same clone as 60626-1-PBS)

    IHC staining of human tonsillitis using 60626-1-Ig (same clone as 60626-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60626-1-Ig (P16-INK4A antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60626-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CDKN2A/P16INK4A antibody included

P16-INK4A Monoclonal antibody, PBS Only

P16-INK4A Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 60626-1-PBS

产品说明书

CloneNo. 1C9D3

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

p16, p16 INK4, P16 INK4A, p16-INK4, p16INK4A

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy CDKN2A/P16INK4A Ready-To-Use IHC Kit. CDKN2A/P16INK4A primary antibody included.

产品信息

60626-1-PBS targets P16-INK4A in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原 P16-INK4A fusion protein Ag1328 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 cyclin-dependent kinase inhibitor 2A
别名 p16, p16 INK4, P16 INK4A, p16-INK4, p16INK4A
计算分子量 16 kDa
观测分子量 16 kDa
GenBank蛋白编号BC021998
基因名称 CDKN2A
Gene ID (NCBI) 1029
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP42771
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

P16-INK4A is also named as CDKN2A, MLM, Tumor suppressor ARF, Alternative reading frame. The tumor suppressor protein p16Ink4a (encoded from the CDKN2A locus) is often transcriptionally activated in cells undergoing senescence and is one of the main regulators of this program, and it is upregulated in multiple tissues during aging (PMID:17055429). p16-Ink4a is the principal member of the Ink4 family of CDK inhibitors. p16-Ink4a contributes to the regulation of cell cycle progression by inhibiting the S phase. p16Ink4a binds to CDK4/6, inhibiting cyclin D-CDK4/6 complex formation and CDK4/6-mediated phosphorylation of Rb family members. Expression of p16-Ink4a maintains the Rb family members in a hypophosphorylated state, which promotes binding to E2F1 and leads to G1 cell cycle arrest (PMID: 21297668).