验证数据展示
经过测试的应用
Positive WB detected in | A549 cells, MCF-7 cells, HCT 116 cells, HUVEC cells, HeLa cells, HepG2 cells, THP-1 cells, U-87 MG cells |
Positive IP detected in | MCF-7 cells |
Positive IF/ICC detected in | MCF-7 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:3000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunofluorescence (IF)/ICC | IF/ICC : 1:1000-1:4000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 1 publications below |
产品信息
82669-2-RR targets P21 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, IF/ICC, IP, ELISA Application Description |
文献引用应用 | WB |
经测试反应性 | human |
文献引用反应性 | human |
免疫原 | P21 fusion protein Ag0368 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | cyclin-dependent kinase inhibitor 1A (p21, Cip1) |
别名 | CDKN1A, P21;CDKN1A, 3N14, CDK-interacting protein 1, CIP1 |
计算分子量 | 18 kDa |
观测分子量 | 21 kDa |
GenBank蛋白编号 | BC000275 |
基因名称 | p21 |
Gene ID (NCBI) | 1026 |
RRID | AB_3086498 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | P38936 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CDKN1A (p21, CIP1, WAF1) is a cyclin-dependent kinase inhibitor. CDKN1A binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at the G1 phase. The expression of CDKN1A is induced by wild-type but not mutant p53 protein, through which CDKN1A mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. CDKN1A can interact with proliferating cell nuclear antigen (PCNA), and plays a regulatory role in S phase DNA replication and DNA damage repair. CDKN1A was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Two alternatively spliced variants, which encode an identical protein, have been reported.
实验方案
Product Specific Protocols | |
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WB protocol for P21 antibody 82669-2-RR | Download protocol |
IF protocol for P21 antibody 82669-2-RR | Download protocol |
IP protocol for P21 antibody 82669-2-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |