P21 Recombinant antibody

P21 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, IP, ELISA
Cat No. 82669-2-RR

产品说明书

CloneNo. 3N14

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, IP, ELISA

CDKN1A, P21;CDKN1A, 3N14, CDK-interacting protein 1, CIP1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive WB detected inA549 cells, MCF-7 cells, HCT 116 cells, HUVEC cells, HeLa cells, HepG2 cells, THP-1 cells, U-87 MG cells
Positive IP detected inMCF-7 cells
Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy CDKN1A/P21 Ready-To-Use IHC Kit. CDKN1A/P21 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

82669-2-RR targets P21 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原 P21 fusion protein Ag0368 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cyclin-dependent kinase inhibitor 1A (p21, Cip1)
别名 CDKN1A, P21;CDKN1A, 3N14, CDK-interacting protein 1, CIP1
计算分子量18 kDa
观测分子量21 kDa
GenBank蛋白编号BC000275
基因名称 p21
Gene ID (NCBI) 1026
RRIDAB_3086498
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP38936
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CDKN1A (p21, CIP1, WAF1) is a cyclin-dependent kinase inhibitor. CDKN1A binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at the G1 phase. The expression of CDKN1A is induced by wild-type but not mutant p53 protein, through which CDKN1A mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. CDKN1A can interact with proliferating cell nuclear antigen (PCNA), and plays a regulatory role in S phase DNA replication and DNA damage repair. CDKN1A was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Two alternatively spliced variants, which encode an identical protein, have been reported.

实验方案

Product Specific Protocols
WB protocol for P21 antibody 82669-2-RRDownload protocol
IF protocol for P21 antibody 82669-2-RRDownload protocol
IP protocol for P21 antibody 82669-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Biol Chem

The P53-P21-RB1 pathway promotes BRD4 degradation in liver cancer through USP1

Authors - Neng Li
Loading...