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验证数据展示

  • WB analysis using 82669-2-RR (same clone as 82669-2-PBS)

    WB analysis using 82669-2-RR (same clone as 82669-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82669-2-RR (P21 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82669-2-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 82669-2-RR (same clone as 82669-2-PBS)

    IF Staining of MCF-7 using 82669-2-RR (same clone as 82669-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using P21 antibody (82669-2-RR, Clone: 3N14 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82669-2-PBS in a different storage buffer formulation.

  • IP experiment of MCF-7 using 82669-2-RR (same clone as 82669-2-PBS)

    IP experiment of MCF-7 using 82669-2-RR (same clone as 82669-2-PBS)

    IP result of anti-P21 (IP:82669-2-RR, 4ug; Detection:82669-2-RR 1:800) with MCF-7 cells lysate 760 ug. This data was developed using the same antibody clone with 82669-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82669-2-RR

    Affinity and Kinetic Characterization of 82669-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82669-2-RR against Human P21 were performed. The affinity constant is 112 nM.

P21 Recombinant antibody, PBS Only

P21 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 82669-2-PBS

产品说明书

CloneNo. 3N14

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, IP, Indirect ELISA

别名

CDKN1A, P21;CDKN1A, 3N14, CDK-interacting protein 1, CIP1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy CDKN1A/P21 Ready-To-Use IHC Kit. CDKN1A/P21 primary antibody included.

产品信息

82669-2-PBS targets P21 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原 P21 fusion protein Ag0368 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cyclin-dependent kinase inhibitor 1A (p21, Cip1)
别名 CDKN1A, P21;CDKN1A, 3N14, CDK-interacting protein 1, CIP1
计算分子量18 kDa
观测分子量21 kDa
GenBank蛋白编号BC000275
基因名称 p21
Gene ID (NCBI) 1026
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP38936
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CDKN1A (p21, CIP1, WAF1) is a cyclin-dependent kinase inhibitor. CDKN1A binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at the G1 phase. The expression of CDKN1A is induced by wild-type but not mutant p53 protein, through which CDKN1A mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. CDKN1A can interact with proliferating cell nuclear antigen (PCNA), and plays a regulatory role in S phase DNA replication and DNA damage repair. CDKN1A was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Two alternatively spliced variants, which encode an identical protein, have been reported.