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P21 Recombinant antibody, PBS Only

P21 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3N14

Cat no : 82669-2-PBS

Print datasheet

Synonyms

CDKN1A, P21;CDKN1A, Cyclin-dependent kinase inhibitor 1, Cyclin dependent kinase inhibitor 1, CIP1

验证数据展示

  • WB analysis using 82669-2-RR (same clone as 82669-2-PBS)

    WB analysis using 82669-2-RR (same clone as 82669-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82669-2-RR (P21 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82669-2-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 82669-2-RR (same clone as 82669-2-PBS)

    IF Staining of MCF-7 using 82669-2-RR (same clone as 82669-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using P21 antibody (82669-2-RR, Clone: 3N14 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82669-2-PBS in a different storage buffer formulation.

  • IP experiment of MCF-7 using 82669-2-RR (same clone as 82669-2-PBS)

    IP experiment of MCF-7 using 82669-2-RR (same clone as 82669-2-PBS)

    IP result of anti-P21 (IP:82669-2-RR, 4ug; Detection:82669-2-RR 1:800) with MCF-7 cells lysate 760 ug. This data was developed using the same antibody clone with 82669-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82669-2-RR

    Affinity and Kinetic Characterization of 82669-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82669-2-RR against Human P21 were performed. The affinity constant is 112 nM.

Planning an IHC experiment? We recommend our IHCeasy CDKN1A/P21 Ready-To-Use IHC Kit. CDKN1A/P21 primary antibody included.

产品信息

82669-2-PBS targets P21 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF/ICC, IP, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen P21 fusion protein Ag0368 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name cyclin-dependent kinase inhibitor 1A (p21, Cip1)
Synonyms CDKN1A, P21;CDKN1A, Cyclin-dependent kinase inhibitor 1, Cyclin dependent kinase inhibitor 1, CIP1
Calculated Molecular Weight18 kDa
Observed Molecular Weight21 kDa
GenBank Accession NumberBC000275
Gene Symbol p21
Gene ID (NCBI) 1026
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP38936
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CDKN1A (p21, CIP1, WAF1) is a cyclin-dependent kinase inhibitor. CDKN1A binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at the G1 phase. The expression of CDKN1A is induced by wild-type but not mutant p53 protein, through which CDKN1A mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. CDKN1A can interact with proliferating cell nuclear antigen (PCNA), and plays a regulatory role in S phase DNA replication and DNA damage repair. CDKN1A was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Two alternatively spliced variants, which encode an identical protein, have been reported.