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  • Featured Product
  • KD/KO Validated

P62,SQSTM1 Monoclonal antibody, PBS Only

P62,SQSTM1 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1H5C1

Cat no : 66184-1-PBS

Print datasheet

Synonyms

A170, EBIAP, ORCA, OSIL, p60, P62, P62/SQSTM1, P62;SQSTM1, p62B, PDB3, sequestosome 1, SQSTM1, Ubiquitin binding protein p62, ZIP3

验证数据展示

  • WB analysis using 66184-1-Ig (same clone as 66184-1-PBS)

    WB analysis using 66184-1-Ig (same clone as 66184-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66184-1-Ig (P62,SQSTM1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66184-1-PBS in a different storage buffer formulation.

  • WB analysis of U2OS using 66184-1-Ig (same clone as 66184-1-PBS)

    WB analysis of U2OS using 66184-1-Ig (same clone as 66184-1-PBS)

    U2OS (WT and SQSTM1 KO) lysates prepared with RIPA buffer, 25 μg protein loaded. 66184-1-Ig incubated at 1:1000 at 4°C overnight in 5% milk in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency. This data was developed using the same antibody clone with 66184-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 66184-1-Ig (same clone as 66184-1-PBS)

    WB analysis of HEK-293 using 66184-1-Ig (same clone as 66184-1-PBS)

    WB result of P62,SQSTM1 antibody (66184-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-P62/SQSTM1 transfected HEK-293 cells. This data was developed using the same antibody clone with 66184-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 66184-1-Ig (same clone as 66184-1-PBS)

    IF Staining of U2OS using 66184-1-Ig (same clone as 66184-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using P62/SQSTM1 antibody (66184-1-Ig, Clone: 1H5C1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66184-1-PBS in a different storage buffer formulation.

  • IP experiment of U2OS using 66184-1-Ig (same clone as 66184-1-PBS)

    IP experiment of U2OS using 66184-1-Ig (same clone as 66184-1-PBS)

    U2OS lysates prepared and IP of SQSTM1 performed using 1.0 μg of 66184-1-Ig coupled to protein G- Sepharose beads. The Ponceau stained transfers of each blot are shown. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency. This data was developed using the same antibody clone with 66184-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 66184-1-Ig (same clone as 66184-1-PBS)

    IHC staining of human lung cancer using 66184-1-Ig (same clone as 66184-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66184-1-Ig (P62,SQSTM1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66184-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with P62,SQSTM1 antibody included
Planning an IHC experiment? We recommend our IHCeasy P62,SQSTM1 Ready-To-Use IHC Kit. P62,SQSTM1 primary antibody included.

产品信息

66184-1-PBS targets P62,SQSTM1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
Tested Reactivity human
Immunogen P62,SQSTM1 fusion protein Ag13131 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name sequestosome 1
Synonyms A170, EBIAP, ORCA, OSIL, p60, P62, P62/SQSTM1, P62;SQSTM1, p62B, PDB3, sequestosome 1, SQSTM1, Ubiquitin binding protein p62, ZIP3
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 62 kDa
GenBank Accession NumberBC017222
Gene Symbol P62/SQSTM1
Gene ID (NCBI) 8878
RRIDAB_2881579
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13501
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Sequestosome 1 (SQSTM1/p62) is a multifunctional adaptor protein implicated in selective autophagy, cell signaling pathways, and tumorigenesis. p62 has been implicated in shuttling ubiquitinated and sometimes aggregated proteins for autophagic degradation. As a autophagy-specific substrate, p62 is degraded during the autophagic process, which makes intracellular level of p62 as a marker for autophagy flux. p62 is at the cross-roads of several signaling pathways including Ras/ Raf/ MAPK and NFκB and plays important role in cancer. p62 is a component of inclusion bodies/ protein aggregates found in human diseases, including Huntington's disease, Alzheimer's disease, Parkinson's disease in the brain, and nephropathic cystinosis in kidney (PMID: 22074114, 22860231, 22714671). The molecular weight of p62 is predicted as 48/ 38 kDa, while western blot analyses using this antibody demonstrate the major band around 60-62 kDa in various tissues.