验证数据展示
产品信息
66184-1-PBS targets P62/SQSTM1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.
经测试应用 | WB, IHC, IF/ICC, IP, Indirect ELISA Application Description |
经测试反应性 | human |
免疫原 | P62/SQSTM1 fusion protein Ag13131 种属同源性预测 |
宿主/亚型 | Mouse / IgG2b |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | sequestosome 1 |
别名 | P62,SQSTM1, P62, SQSTM1, 1H5C1, EBI3-associated protein of 60 kDa |
计算分子量 | 48 kDa |
观测分子量 | 62 kDa |
GenBank蛋白编号 | BC017222 |
基因名称 | P62/SQSTM1 |
Gene ID (NCBI) | 8878 |
RRID | AB_2881579 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | Q13501 |
储存缓冲液 | PBS only , pH 7.3 |
储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
Sequestosome 1 (SQSTM1/p62) is a multifunctional adaptor protein implicated in selective autophagy, cell signaling pathways, and tumorigenesis. p62 has been implicated in shuttling ubiquitinated and sometimes aggregated proteins for autophagic degradation. As a autophagy-specific substrate, p62 is degraded during the autophagic process, which makes intracellular level of p62 as a marker for autophagy flux. p62 is at the cross-roads of several signaling pathways including Ras/ Raf/ MAPK and NFκB and plays important role in cancer. p62 is a component of inclusion bodies/ protein aggregates found in human diseases, including Huntington's disease, Alzheimer's disease, Parkinson's disease in the brain, and nephropathic cystinosis in kidney (PMID: 22074114, 22860231, 22714671). The molecular weight of p62 is predicted as 48/ 38 kDa, while western blot analyses using this antibody demonstrate the major band around 60-62 kDa in various tissues.