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P62,SQSTM1 Monoclonal antibody

P62,SQSTM1 Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human and More (3)

Applications

WB, IHC, IF/ICC, IP, ELISA and More (2)

Conjugate

Unconjugated

CloneNo.

1H5C1

Cat no : 66184-1-Ig

Print datasheet

Synonyms

A170, EBIAP, ORCA, OSIL, p60, P62, P62/SQSTM1, P62;SQSTM1, p62B, PDB3, sequestosome 1, SQSTM1, Ubiquitin binding protein p62, ZIP3



经过测试的应用

Positive WB detected inHeLa cells, U2OS cells, K-562 cells, HEK-293 cells, MCF-7 cells, HepG2 cells, Jurkat cells, L02 cells, Raji cells
Positive IP detected inU2OS cells
Positive IHC detected inhuman lung cancer tissue, human colon cancer tissue, human liver cancer tissue, human endometrial cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells, Chloroquine treated U2OS cells, Starvation treated HepG2 cells
Planning an IHC experiment? We recommend our IHCeasy P62,SQSTM1 Ready-To-Use IHC Kit. P62,SQSTM1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66184-1-Ig targets P62,SQSTM1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP, CoIP
Tested Reactivity human
Cited Reactivityhuman, pig, monkey, bovine
Immunogen P62,SQSTM1 fusion protein Ag13131 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name sequestosome 1
Synonyms A170, EBIAP, ORCA, OSIL, p60, P62, P62/SQSTM1, P62;SQSTM1, p62B, PDB3, sequestosome 1, SQSTM1, Ubiquitin binding protein p62, ZIP3
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 62 kDa
GenBank Accession NumberBC017222
Gene Symbol P62/SQSTM1
Gene ID (NCBI) 8878
RRIDAB_2881579
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13501
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Sequestosome 1 (SQSTM1/p62) is a multifunctional adaptor protein implicated in selective autophagy, cell signaling pathways, and tumorigenesis. p62 has been implicated in shuttling ubiquitinated and sometimes aggregated proteins for autophagic degradation. As a autophagy-specific substrate, p62 is degraded during the autophagic process, which makes intracellular level of p62 as a marker for autophagy flux. p62 is at the cross-roads of several signaling pathways including Ras/ Raf/ MAPK and NFκB and plays important role in cancer. p62 is a component of inclusion bodies/ protein aggregates found in human diseases, including Huntington's disease, Alzheimer's disease, Parkinson's disease in the brain, and nephropathic cystinosis in kidney (PMID: 22074114, 22860231, 22714671). The molecular weight of p62 is predicted as 48/ 38 kDa, while western blot analyses using this antibody demonstrate the major band around 60-62 kDa in various tissues.

实验方案

Product Specific Protocols
WB protocol for P62,SQSTM1 antibody 66184-1-IgDownload protocol
IHC protocol for P62,SQSTM1 antibody 66184-1-IgDownload protocol
IF protocol for P62,SQSTM1 antibody 66184-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Gastroenterology

Proteomic characterization identifies clinically relevant subgroups of gastrointestinal stromal tumors

Authors - Mingjun Sun
humanIHC,IF

Acta Neuropathol

Upstream open reading frame with NOTCH2NLC GGC expansion generates polyglycine aggregates and disrupts nucleocytoplasmic transport: implications for polyglycine diseases.

Authors - Shaoping Zhong
humanWB

Nat Commun

IL-6 regulates autophagy and chemotherapy resistance by promoting BECN1 phosphorylation.

Authors - Fuqing Hu
IHC

Mol Neurodegener

Collusion of α-Synuclein and Aβ aggravating co-morbidities in a novel prion-type mouse model

Authors - Grace M. Lloyd
humanWB

J Cell Biol

CHMP2B regulates TDP-43 phosphorylation and cytotoxicity independent of autophagy via CK1.

Authors - Xue Deng
humanWB

Theranostics

The c-Myc/miR-27b-3p/ATG10 regulatory axis regulates chemoresistance in colorectal cancer.

Authors - Wu Sun