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P62,SQSTM1 Monoclonal antibody
P62,SQSTM1 Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human and More (3)
Applications
WB, IHC, IF/ICC, IP, ELISA and More (2)
Conjugate
Unconjugated
134
CloneNo.
1H5C1
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, U2OS cells, K-562 cells, HEK-293 cells, MCF-7 cells, HepG2 cells, Jurkat cells, L02 cells, Raji cells |
Positive IP detected in | U2OS cells |
Positive IHC detected in | human lung cancer tissue, human colon cancer tissue, human liver cancer tissue, human endometrial cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | U2OS cells, Chloroquine treated U2OS cells, Starvation treated HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
66184-1-Ig targets P62,SQSTM1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.
Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
Cited Applications | WB, IHC, IF, IP, CoIP |
Tested Reactivity | human |
Cited Reactivity | human, pig, monkey, bovine |
Immunogen | P62,SQSTM1 fusion protein Ag13131 种属同源性预测 |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Full Name | sequestosome 1 |
Synonyms | A170, EBIAP, ORCA, OSIL, p60, P62, P62/SQSTM1, P62;SQSTM1, p62B, PDB3, sequestosome 1, SQSTM1, Ubiquitin binding protein p62, ZIP3 |
Calculated Molecular Weight | 48 kDa |
Observed Molecular Weight | 62 kDa |
GenBank Accession Number | BC017222 |
Gene Symbol | P62/SQSTM1 |
Gene ID (NCBI) | 8878 |
RRID | AB_2881579 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q13501 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for P62,SQSTM1 antibody 66184-1-Ig | Download protocol |
IHC protocol for P62,SQSTM1 antibody 66184-1-Ig | Download protocol |
IF protocol for P62,SQSTM1 antibody 66184-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Gastroenterology Proteomic characterization identifies clinically relevant subgroups of gastrointestinal stromal tumors | ||
Acta Neuropathol Upstream open reading frame with NOTCH2NLC GGC expansion generates polyglycine aggregates and disrupts nucleocytoplasmic transport: implications for polyglycine diseases. | ||
Nat Commun IL-6 regulates autophagy and chemotherapy resistance by promoting BECN1 phosphorylation. | ||
Mol Neurodegener Collusion of α-Synuclein and Aβ aggravating co-morbidities in a novel prion-type mouse model | ||
J Cell Biol CHMP2B regulates TDP-43 phosphorylation and cytotoxicity independent of autophagy via CK1. | ||
Theranostics The c-Myc/miR-27b-3p/ATG10 regulatory axis regulates chemoresistance in colorectal cancer. |