• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • IF Staining of HepG2 using CL488-66184

    IF Staining of HepG2 using CL488-66184

    Immunofluorescent analysis of (-20°C Ethanol) fixed Chloroquine treated HepG2 cells using CL488-66184 (P62,SQSTM1 antibody) at dilution of 1:100. .

  • IF Staining of HepG2 using CL488-66184

    IF Staining of HepG2 using CL488-66184

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL488-66184 (P62,SQSTM1 antibody) at dilution of 1:100.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated P62/SQSTM1 Monoclonal antibody

P62/SQSTM1 Monoclonal Antibody for IF/ICC
Cat No. CL488-66184

产品说明书

CloneNo. 1H5C1

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

IF/ICC

别名

P62,SQSTM1, P62, SQSTM1, 1H5C1, EBI3-associated protein of 60 kDa

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive IF/ICC detected inHepG2 cells, Chloroquine treated U2OS cells
Planning an IHC experiment? We recommend our IHCeasy P62,SQSTM1 Ready-To-Use IHC Kit. P62,SQSTM1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66184-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66184 targets P62/SQSTM1 in IF/ICC applications and shows reactivity with human samples.

经测试应用 IF/ICC Application Description
经测试反应性 human
免疫原 P62/SQSTM1 fusion protein Ag13131 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 sequestosome 1
别名 P62,SQSTM1, P62, SQSTM1, 1H5C1, EBI3-associated protein of 60 kDa
计算分子量 48 kDa
观测分子量 62 kDa
GenBank蛋白编号BC017222
基因名称 P62/SQSTM1
Gene ID (NCBI) 8878
RRIDAB_2883277
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ13501
储存缓冲液 PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Sequestosome 1 (SQSTM1/p62) is a multifunctional adaptor protein implicated in selective autophagy, cell signaling pathways, and tumorigenesis. p62 has been implicated in shuttling ubiquitinated and sometimes aggregated proteins for autophagic degradation. As a autophagy-specific substrate, p62 is degraded during the autophagic process, which makes intracellular level of p62 as a marker for autophagy flux. p62 is at the cross-roads of several signaling pathways including Ras/ Raf/ MAPK and NFκB and plays important role in cancer. p62 is a component of inclusion bodies/ protein aggregates found in human diseases, including Huntington's disease, Alzheimer's disease, Parkinson's disease in the brain, and nephropathic cystinosis in kidney (PMID: 22074114, 22860231, 22714671). The molecular weight of p62 is predicted as 48/ 38 kDa, while western blot analyses using this antibody demonstrate the major band around 60-62 kDa in various tissues.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 P62/SQSTM1 antibody CL488-66184Download protocol
Standard Protocols
Click here to view our Standard Protocols