验证数据展示
经过测试的应用
| Positive WB detected in | NIH/3T3 cells, HeLa cells, mouse brain tissue, rat brain tissue, HepG2 cells, Jurkat cells, Raji cells, HEK-293T cells, MCF-7 cells, mouse liver tissue |
| Positive IP detected in | NIH/3T3 cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | NIH/3T3 cells |
| Positive FC (Intra) detected in | NIH/3T3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
84826-1-RR targets P62/SQSTM1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | P62/SQSTM1 fusion protein Ag35064 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | sequestosome 1 |
| 别名 | p62, Sqstm1, 241992C4, OSF 6, Osi |
| 计算分子量 | 48 kDa |
| 观测分子量 | 62 kDa |
| GenBank蛋白编号 | NM_011018 |
| 基因名称 | P62/SQSTM1 |
| Gene ID (NCBI) | 18412 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q64337 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Sequestosome 1 (SQSTM1/p62) is a multifunctional adaptor protein implicated in selective autophagy, cell signaling pathways, and tumorigenesis. p62 has been implicated in shuttling ubiquitinated and aggregated proteins for autophagic degradation. p62 is degraded during the autophagic process, which makes its intracellular level a marker for autophagy progression. p62 is at the cross-roads of several signaling pathways including Ras/ Raf/ MAPK and NFκB and plays important role in cancer.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for P62/SQSTM1 antibody 84826-1-RR | Download protocol |
| IHC protocol for P62/SQSTM1 antibody 84826-1-RR | Download protocol |
| IF protocol for P62/SQSTM1 antibody 84826-1-RR | Download protocol |
| IP protocol for P62/SQSTM1 antibody 84826-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |