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  • Featured Product
  • KD/KO Validated

PA2G4 Monoclonal antibody, PBS Only (Detector)

PA2G4 Monoclonal Antibody for WB, IF, IHC, Sandwich ELISA, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, rat, mouse

Applications

WB, IF, IHC, Sandwich ELISA, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3G10D9

Cat No : 66055-1-PBS

Print datasheet

Synonyms

EBP1, ErbB3 binding protein 1, HG4 1, p38 2G4, PA2G4

验证数据展示

  • WB analysis using 66055-1-Ig (same clone as 66055-1-PBS)

    WB analysis using 66055-1-Ig (same clone as 66055-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66055-1-Ig (PA2G4 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66055-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 66055-1-Ig (same clone as 66055-1-PBS)

    WB analysis of HEK-293 using 66055-1-Ig (same clone as 66055-1-PBS)

    WB result of PA2G4 antibody (66055-1-Ig; 1:100000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PA2G4 transfected HEK-293 cells. This data was developed using the same antibody clone with 66055-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66055-1-Ig (same clone as 66055-1-PBS)

    IF Staining of HeLa using 66055-1-Ig (same clone as 66055-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PA2G4 antibody (66055-1-Ig, Clone: 3G10D9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66055-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66055-1-Ig (same clone as 66055-1-PBS)

    IHC staining of human breast cancer using 66055-1-Ig (same clone as 66055-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66055-1-Ig (PA2G4 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66055-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PA2G4 antibody included
  • Sandwich ELISA standard curve of MP50029-1

    Sandwich ELISA standard curve of MP50029-1

    Sandwich ELISA standard curve of MP50029-1, PA2G4 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66055-2-PBS. Detection antibody: HRP-conjugated 66055-1-PBS. Standard: Ag7850. Range: 1.25-20 ng/mL.

Planning an IHC experiment? We recommend our IHCeasy PA2G4 Ready-To-Use IHC Kit. PA2G4 primary antibody included.

产品信息

66055-1-PBS targets PA2G4 as part of a matched antibody pair:

MP50029-1: 66055-2-PBS capture and 66055-1-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IF, IHC, Sandwich ELISA, Indirect ELISA Application Description
Tested Reactivity human, rat, mouse
Immunogen PA2G4 fusion protein Ag7850 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name proliferation-associated 2G4, 38kDa
Synonyms EBP1, ErbB3 binding protein 1, HG4 1, p38 2G4, PA2G4
Calculated Molecular Weight 44 kDa
Observed Molecular Weight 38-42 kDa, 48 kDa
GenBank Accession NumberBC001951
Gene Symbol PA2G4
Gene ID (NCBI) 5036
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UQ80
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

PA2G4 (EBP1), first identified as an ErbB3 binding protein, is a 38 kDa protein that is widely expressed in cultured cells and tissues. The presence of several functional domains such as a nuclear localization sequence (NLS), LxxLL and LxCxE suggests the involvement of Ebp1 in cell signaling pathways and gene transcription regulation. This antibody is a mouse mono-clonal antibody raised against full length PA2G4 of human origin.