验证数据展示
经过测试的应用
| Positive WB detected in | PC-3 cells, HEK-293 cells, LNCaP cells, HeLa cells, Jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy PA2G4 Ready-To-Use IHC Kit. PA2G4 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:20000-1:100000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
66055-1-Ig targets PA2G4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat, mouse samples.
| Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human, rat, mouse |
| Cited Reactivity | human |
| Immunogen | PA2G4 fusion protein Ag7850 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | proliferation-associated 2G4, 38kDa |
| Synonyms | EBP1, ErbB3 binding protein 1, HG4 1, p38 2G4, PA2G4 |
| Calculated Molecular Weight | 44 kDa |
| Observed Molecular Weight | 38-42 kDa, 48 kDa |
| GenBank Accession Number | BC001951 |
| Gene Symbol | PA2G4 |
| Gene ID (NCBI) | 5036 |
| RRID | AB_11042597 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9UQ80 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PA2G4 (EBP1), first identified as an ErbB3 binding protein, is a 38 kDa protein that is widely expressed in cultured cells and tissues. The presence of several functional domains such as a nuclear localization sequence (NLS), LxxLL and LxCxE suggests the involvement of Ebp1 in cell signaling pathways and gene transcription regulation. This antibody is a mouse mono-clonal antibody raised against full length PA2G4 of human origin.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PA2G4 antibody 66055-1-Ig | Download protocol |
| IHC protocol for PA2G4 antibody 66055-1-Ig | Download protocol |
| IF protocol for PA2G4 antibody 66055-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Stem Cell HectD1 controls hematopoietic stem cell regeneration by coordinating ribosome assembly and protein synthesis. | ||
Nat Commun Orthogonal proteogenomic analysis identifies the druggable PA2G4-MYC axis in 3q26 AML |
