验证数据展示
经过测试的应用
Positive WB detected in | PC-3 cells, HEK-293 cells, LNCaP cells, HeLa cells, Jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells |
Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:20000-1:100000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 2 publications below |
产品信息
66055-1-Ig targets PA2G4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat, mouse samples.
Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
Cited Applications | WB |
Tested Reactivity | human, rat, mouse |
Cited Reactivity | human |
Immunogen | PA2G4 fusion protein Ag7850 种属同源性预测 |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Full Name | proliferation-associated 2G4, 38kDa |
Synonyms | EBP1, ErbB3 binding protein 1, HG4 1, p38 2G4, PA2G4 |
Calculated Molecular Weight | 44 kDa |
Observed Molecular Weight | 38-42 kDa, 48 kDa |
GenBank Accession Number | BC001951 |
Gene Symbol | PA2G4 |
Gene ID (NCBI) | 5036 |
RRID | AB_11042597 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q9UQ80 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PA2G4 (EBP1), first identified as an ErbB3 binding protein, is a 38 kDa protein that is widely expressed in cultured cells and tissues. The presence of several functional domains such as a nuclear localization sequence (NLS), LxxLL and LxCxE suggests the involvement of Ebp1 in cell signaling pathways and gene transcription regulation. This antibody is a mouse mono-clonal antibody raised against full length PA2G4 of human origin.
实验方案
Product Specific Protocols | |
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WB protocol for PA2G4 antibody 66055-1-Ig | Download protocol |
IHC protocol for PA2G4 antibody 66055-1-Ig | Download protocol |
IF protocol for PA2G4 antibody 66055-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell Stem Cell HectD1 controls hematopoietic stem cell regeneration by coordinating ribosome assembly and protein synthesis. | ||
Nat Commun Orthogonal proteogenomic analysis identifies the druggable PA2G4-MYC axis in 3q26 AML |