验证数据展示
经过测试的应用
Positive IF/ICC detected in | HeLa cells |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL594-66055 targets PA2G4 in IF/ICC applications and shows reactivity with human, rat, mouse samples.
经测试应用 | IF/ICC Application Description |
经测试反应性 | human, rat, mouse |
免疫原 | PA2G4 fusion protein Ag7850 种属同源性预测 |
宿主/亚型 | Mouse / IgG2a |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | proliferation-associated 2G4, 38kDa |
别名 | EBP1, ErbB3 binding protein 1, HG4 1, p38 2G4, PA2G4 |
计算分子量 | 44 kDa |
观测分子量 | 38-42 kDa, 48 kDa |
GenBank蛋白编号 | BC001951 |
基因名称 | PA2G4 |
Gene ID (NCBI) | 5036 |
RRID | AB_2883494 |
偶联类型 | CoraLite®594 Fluorescent Dye |
最大激发/发射波长 | 588 nm / 604 nm |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | Q9UQ80 |
储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PA2G4 (EBP1), first identified as an ErbB3 binding protein, is a 38 kDa protein that is widely expressed in cultured cells and tissues. The presence of several functional domains such as a nuclear localization sequence (NLS), LxxLL and LxCxE suggests the involvement of Ebp1 in cell signaling pathways and gene transcription regulation. This antibody is a mouse mono-clonal antibody raised against full length PA2G4 of human origin.
实验方案
Product Specific Protocols | |
---|---|
IF protocol for CL594 PA2G4 antibody CL594-66055 | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |