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验证数据展示

  • IF Staining of HeLa using CL488-66055

    IF Staining of HeLa using CL488-66055

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 PA2G4 antibody (CL488-66055, Clone: 3G10D9 ) at dilution of 1:200.

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  • KD/KO验证

CoraLite® Plus 488-conjugated PA2G4 Monoclonal antibody

PA2G4 Monoclonal Antibody for IF/ICC
Cat No. CL488-66055

产品说明书

CloneNo. 3G10D9

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat

应用

IF/ICC

别名

HG4 1, ErbB3-binding protein 1, ErbB3 binding protein 1, EBP1, Cell cycle protein p38-2G4 homolog

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PA2G4 Ready-To-Use IHC Kit. PA2G4 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66055-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66055 targets PA2G4 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, rat
免疫原 PA2G4 fusion protein Ag7850 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 proliferation-associated 2G4, 38kDa
别名 HG4 1, ErbB3-binding protein 1, ErbB3 binding protein 1, EBP1, Cell cycle protein p38-2G4 homolog
计算分子量 44 kDa
观测分子量 38-42 kDa, 48 kDa
GenBank蛋白编号BC001951
基因名称 PA2G4
Gene ID (NCBI) 5036
RRIDAB_2883242
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9UQ80
储存缓冲液 PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PA2G4 (EBP1), first identified as an ErbB3 binding protein, is a 38 kDa protein that is widely expressed in cultured cells and tissues. The presence of several functional domains such as a nuclear localization sequence (NLS), LxxLL and LxCxE suggests the involvement of Ebp1 in cell signaling pathways and gene transcription regulation. This antibody is a mouse mono-clonal antibody raised against full length PA2G4 of human origin.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 PA2G4 antibody CL488-66055Download protocol
Standard Protocols
Click here to view our Standard Protocols