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验证数据展示

  • IF Staining of HEK-293 using CL488-66809

    IF Staining of HEK-293 using CL488-66809

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CL488-66809 (PABPC1,PABP antibody) at dilution of 1:100.

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated PABPC1,PABP Monoclonal antibody

PABPC1,PABP Monoclonal Antibody for IF/ICC
Cat No. CL488-66809

Print datasheet

CloneNo. 3D8B5

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat

Applications

IF/ICC

Synonyms

PAB1, PABP, PABP 1, PABP1, PABPC1, PABPC2, PABPL1, Poly(A) binding protein 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy PABPC1 Ready-To-Use IHC Kit. PABPC1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66809-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66809 targets PABPC1,PABP in IF/ICC applications and shows reactivity with Human, rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human, rat
Immunogen PABPC1,PABP fusion protein Ag27860 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name poly(A) binding protein, cytoplasmic 1
Synonyms PAB1, PABP, PABP 1, PABP1, PABPC1, PABPC2, PABPL1, Poly(A) binding protein 1
Calculated Molecular Weight 71 kDa
Observed Molecular Weight 71 kDa
GenBank Accession NumberBC015958
Gene Symbol PABPC1
Gene ID (NCBI) 26986
RRIDAB_2883384
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP11940
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The poly(A)-binding protein (PABP), which is found complexed to the 3-prime poly(A) tail of eukaryotic mRNA, is required for poly(A) shortening and translation initiation [PMID:21989405]. Polyadenylate-binding protein 1 (PABPC1) is a cytoplasmic-nuclear shuttling protein important for protein translation initiation, and both RNA processing and stability. In the cytoplasm, PABPC1 binds to the 3' poly(A) tail of eukaryotic mRNAs through its RNA-recognition motifs (RRM) and interacts with the N-terminus of eIF4G, part of the eIF4F complex associated with the 5' cap structure [PMID:20009508, 17381337].

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 PABPC1,PABP antibody CL488-66809Download protocol
Standard Protocols
Click here to view our Standard Protocols