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  • Featured Product
  • KD/KO Validated

PABPC1,PABP Monoclonal antibody, PBS Only

PABPC1,PABP Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, mouse

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3D8B5

Cat No : 66809-1-PBS

Print datasheet

Synonyms

PAB1, PABP, PABP 1, PABP1, PABPC1, PABPC1,PABP, PABPC2, PABPL1, Poly(A) binding protein 1

验证数据展示

  • WB analysis using 66809-1-Ig (same clone as 66809-1-PBS)

    WB analysis using 66809-1-Ig (same clone as 66809-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66809-1-Ig (PABPC1,PABP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66809-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 66809-1-Ig (same clone as 66809-1-PBS)

    IHC staining of mouse testis using 66809-1-Ig (same clone as 66809-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66809-1-Ig (PABPC1,PABP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66809-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PABPC1 antibody included
  • IHC staining of mouse testis using 66809-1-Ig (same clone as 66809-1-PBS)

    IHC staining of mouse testis using 66809-1-Ig (same clone as 66809-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66809-1-Ig (PABPC1,PABP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66809-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PABPC1 antibody included
  • IF Staining of HEK-293 using 66809-1-Ig (same clone as 66809-1-PBS)

    IF Staining of HEK-293 using 66809-1-Ig (same clone as 66809-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 66809-1-Ig (PABPC1,PABP antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66809-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 66809-1-Ig (same clone as 66809-1-PBS)

    WB analysis of HeLa using 66809-1-Ig (same clone as 66809-1-PBS)

    WB result of PABPC1,PABP antibody (66809-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PABPC1,PABP transfected HeLa cells. This data was developed using the same antibody clone with 66809-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy PABPC1 Ready-To-Use IHC Kit. PABPC1 primary antibody included.

产品信息

66809-1-PBS targets PABPC1,PABP in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Applications WB, IF, IHC, Indirect ELISA Application Description
Tested Reactivity Human, rat, mouse
Immunogen PABPC1,PABP fusion protein Ag27860 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name poly(A) binding protein, cytoplasmic 1
Synonyms PAB1, PABP, PABP 1, PABP1, PABPC1, PABPC1,PABP, PABPC2, PABPL1, Poly(A) binding protein 1
Calculated Molecular Weight 71 kDa
Observed Molecular Weight 71 kDa
GenBank Accession NumberBC015958
Gene Symbol PABPC1
Gene ID (NCBI) 26986
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP11940
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

The poly(A)-binding protein (PABP), which is found complexed to the 3-prime poly(A) tail of eukaryotic mRNA, is required for poly(A) shortening and translation initiation [PMID: 21989405]. Polyadenylate-binding protein 1 (PABPC1) is a cytoplasmic-nuclear shuttling protein important for protein translation initiation, and both RNA processing and stability. In the cytoplasm, PABPC1 binds to the 3' poly(A) tail of eukaryotic mRNAs through its RNA-recognition motifs (RRM) and interacts with the N-terminus of eIF4G, part of the eIF4F complex associated with the 5' cap structure [PMID:20009508, 17381337].