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  • Featured Product
  • KD/KO Validated

PABPC1,PABP Monoclonal antibody, PBS Only

PABPC1,PABP Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3D8B5

Cat no : 66809-1-PBS

Print datasheet

Synonyms

PAB1, PABP, PABP 1, PABP1, PABPC1, PABPC1,PABP, PABPC2, PABPL1, Poly(A) binding protein 1

验证数据展示

  • WB analysis using 66809-1-Ig (same clone as 66809-1-PBS)

    WB analysis using 66809-1-Ig (same clone as 66809-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66809-1-Ig (PABPC1,PABP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66809-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 66809-1-Ig (same clone as 66809-1-PBS)

    IHC staining of mouse testis using 66809-1-Ig (same clone as 66809-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66809-1-Ig (PABPC1,PABP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66809-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PABPC1 antibody included
  • IHC staining of mouse testis using 66809-1-Ig (same clone as 66809-1-PBS)

    IHC staining of mouse testis using 66809-1-Ig (same clone as 66809-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66809-1-Ig (PABPC1,PABP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66809-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PABPC1 antibody included
  • IF Staining of HEK-293 using 66809-1-Ig (same clone as 66809-1-PBS)

    IF Staining of HEK-293 using 66809-1-Ig (same clone as 66809-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 66809-1-Ig (PABPC1,PABP antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66809-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 66809-1-Ig (same clone as 66809-1-PBS)

    WB analysis of HeLa using 66809-1-Ig (same clone as 66809-1-PBS)

    WB result of PABPC1,PABP antibody (66809-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PABPC1,PABP transfected HeLa cells. This data was developed using the same antibody clone with 66809-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy PABPC1 Ready-To-Use IHC Kit. PABPC1 primary antibody included.

产品信息

66809-1-PBS targets PABPC1,PABP in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human, rat, mouse
Immunogen PABPC1,PABP fusion protein Ag27860 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name poly(A) binding protein, cytoplasmic 1
Synonyms PAB1, PABP, PABP 1, PABP1, PABPC1, PABPC1,PABP, PABPC2, PABPL1, Poly(A) binding protein 1
Calculated Molecular Weight 71 kDa
Observed Molecular Weight 71 kDa
GenBank Accession NumberBC015958
Gene Symbol PABPC1
Gene ID (NCBI) 26986
RRIDAB_2882152
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP11940
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The poly(A)-binding protein (PABP), which is found complexed to the 3-prime poly(A) tail of eukaryotic mRNA, is required for poly(A) shortening and translation initiation [PMID: 21989405]. Polyadenylate-binding protein 1 (PABPC1) is a cytoplasmic-nuclear shuttling protein important for protein translation initiation, and both RNA processing and stability. In the cytoplasm, PABPC1 binds to the 3' poly(A) tail of eukaryotic mRNAs through its RNA-recognition motifs (RRM) and interacts with the N-terminus of eIF4G, part of the eIF4F complex associated with the 5' cap structure [PMID:20009508, 17381337].