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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated PABPN1 Monoclonal antibody

PABPN1 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3C12E12

Cat no : CL488-66807

Print datasheet

Synonyms

PABP-2, PABP2, PABII, PAB2, Nuclear poly(A)-binding protein 1

验证数据展示

  • IF Staining of MCF-7 using CL488-66807

    IF Staining of MCF-7 using CL488-66807

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite® Plus 488 PABPN1 antibody (CL488-66807, Clone: 3C12E12 ) at dilution of 1:200.

  • FC experiment of HeLa using CL488-66807

    FC experiment of HeLa using CL488-66807

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human PABPN1 (CL488-66807, Clone:3C12E12) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PABPN1 Ready-To-Use IHC Kit. PABPN1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66807-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66807 targets PABPN1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen PABPN1 fusion protein Ag27470 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name poly(A) binding protein, nuclear 1
Synonyms PABP-2, PABP2, PABII, PAB2, Nuclear poly(A)-binding protein 1
Calculated Molecular Weight 33 kDa
Observed Molecular Weight 50-56 kDa
GenBank Accession NumberBC010939
Gene Symbol PABPN1
Gene ID (NCBI) 8106
RRIDAB_2923756
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ86U42
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Poly(A) Binding Protein Nuclear 1(PABPN1) is one of the isoforms of poly(A) binding protein (PABP) that locating to the nucleus in eukaryotic cells. And PABPN1 plays important role in mRNA stability by regulating of poly-A tail length, mRNA decay and proximal polyadenylation site (PAS) utilization at the 3'-UTR (PMID:23300856). Another report showed that PABPN1 shuttles between the nucleus and the cytoplasm to export poly(A) RNA from the nucleus (PMID:23601051). It is reported that PABPN1 is ubiquitously expressed in almost all tissues, however, mutations in PABPN1 causes Oculopharyngeal Muscular Dystrophy (OPMD) which affected in skeletal muscles specifically. Because of a mild reduction in PABPN1 levels is sufficient to induce muscle wasting (PMID:27152426). Acetylation, Methylation, Phosphoprotein are common post-translational modifications of PABPN1 protein. And 50-55 kDa bands have been reported (PMID: 29939290; 28361972).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 PABPN1 antibody CL488-66807Download protocol
FC protocol for CL Plus 488 PABPN1 antibody CL488-66807Download protocol
Standard Protocols
Click here to view our Standard Protocols