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PACSIN1 Monoclonal antibody

PACSIN1 Monoclonal Antibody for FC, IF/ICC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Rabbit, Chicken, Pig

Applications

FC, IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1B7B4

Cat No : 68115-1-Ig

Print datasheet

Synonyms

KIAA1379, PACSIN1, SDPI, Syndapin-1

验证数据展示

  • WB analysis using 68115-1-Ig

    WB analysis using 68115-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68115-1-Ig (PACSIN1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IHC staining of mouse cerebellum using 68115-1-Ig

    IHC staining of mouse cerebellum using 68115-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 68115-1-Ig (PACSIN1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse cerebellum using 68115-1-Ig

    IHC staining of mouse cerebellum using 68115-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 68115-1-Ig (PACSIN1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of SH-SY5Y using 68115-1-Ig

    IF Staining of SH-SY5Y using 68115-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using PACSIN1 antibody (68115-1-Ig, Clone: 1B7B4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • FC experiment of SH-SY5Y using 68115-1-Ig

    FC experiment of SH-SY5Y using 68115-1-Ig

    1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Anti-Human PACSIN1 (68115-1-Ig, Clone:1B7B4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inpig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue, chicken brain tissue
Positive IHC detected inmouse cerebellum tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSH-SY5Y cells
Positive FC detected inSH-SY5Y cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)IF : 1:400-1:1600
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68115-1-Ig targets PACSIN1 in WB, IF, FC, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat, Rabbit, Chicken, Pig samples.

Tested Applications FC, IF/ICC, IHC, WB, ELISA Application Description
Tested Reactivity Human, Mouse, Rat, Rabbit, Chicken, Pig
Immunogen PACSIN1 fusion protein Ag4102 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name protein kinase C and casein kinase substrate in neurons 1
Synonyms KIAA1379, PACSIN1, SDPI, Syndapin-1
Calculated Molecular Weight 444 aa, 51 kDa
Observed Molecular Weight 48-51 kDa
GenBank Accession NumberBC040228
Gene Symbol PACSIN1
Gene ID (NCBI) 29993
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9BY11
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PACSIN1 (also known as syndapin-1) is a member of the protein kinase C and casein kinase substrate in neurons (PACSIN) family. In mammals, the PACSIN family is comprised of three members, PACSIN1, PACSIN2, and PACSIN3 (PMID: 34990060). PACSIN1 is expressed mainly in neurons, whereas PACSIN2 is ubiquitously expressed in all tissues, and PACSIN3 is expressed mainly in skeletal muscle and the heart (PMID: 23668323). All of these three members contain an N-terminal F-BAR domain and a C-terminal SH3 domain. PACSIN1 plays a role in endocytosis and endosomal recycling. Meanwhile, it has a role in actin remodeling and microtubule nucleation and also plays a particular role in membrane shaping and reconstruction (PMID: 23035120; 34422904). PACSIN1 is involved in neuromorphogenesis and the regulation of the nervous system, and the inappropriate expression of PACSIN1 has been associated with some neurological diseases, including schizophrenia, Alzheimer's disease, and Huntington's disease (PMID: 34990060).

实验方案

Product Specific Protocols
WB protocol for PACSIN1 antibody 68115-1-IgDownload protocol
IHC protocol for PACSIN1 antibody 68115-1-IgDownload protocol
IF protocol for PACSIN1 antibody 68115-1-IgDownload protocol
FC protocol for PACSIN1 antibody 68115-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols