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PACSIN2 Recombinant antibody

PACSIN2 Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

242193H3

Cat no : 84763-4-RR

Print datasheet

Synonyms

Syndapin-II, Syndapin-2, SdpII, Protein kinase C and casein kinase substrate in neurons protein 2, 242193H3

验证数据展示

  • WB analysis using 84763-4-RR

    WB analysis using 84763-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84763-4-RR (PACSIN2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 84763-4-RR

    IF Staining of HepG2 using 84763-4-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PACSIN2 antibody (84763-4-RR, Clone: 242193H3 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84763-4-RR

    Affinity and Kinetic Characterization of 84763-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84763-4-RR against Human PACSIN2 were performed. The affinity constant is 3.72 nM.

经过测试的应用

Positive WB detected inA431 cells, HepG2 cells, NIH/3T3 cells
Positive IF/ICC detected inHepG2 cells, NIH/3T3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84763-4-RR targets PACSIN2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen PACSIN2 fusion protein Ag0809 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name protein kinase C and casein kinase substrate in neurons 2
Synonyms Syndapin-II, Syndapin-2, SdpII, Protein kinase C and casein kinase substrate in neurons protein 2, 242193H3
Calculated Molecular Weight 56 kDa
Observed Molecular Weight60 kDa
GenBank Accession NumberBC008037
Gene Symbol PACSIN2
Gene ID (NCBI) 11252
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9UNF0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Peripheral membrane proteins of the Bin/amphiphysin/Rvs (BAR) and Fer-CIP4 homology-BAR (F-BAR) family participate in cellular membrane trafficking (PMID: 19549836). PACSIN2 (also known as syndapin-2) is a member of the protein kinase C and casein kinase substrate in neurons (PACSIN) family, which are membrane-binding proteins characterized by an amino-terminal F-BAR domain. PACSIN2 plays a role in intracellular vesicle-mediated transport. It is involved in the endocytosis of cell-surface receptors like the EGF receptor, contributing to its internalization (PMID: 23129763). PACSIN2 also has a role in caveolae membrane sculpting (PMID: 21610094).

实验方案

Product Specific Protocols
WB protocol for PACSIN2 antibody 84763-4-RRDownload protocol
IF protocol for PACSIN2 antibody 84763-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols