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PADI4/PAD4 Polyclonal antibody

PADI4/PAD4 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Cat No. 17373-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (2)

PADI4, EC:3.5.3.15, HL 60 PAD, HL-60 PAD, PAD

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive WB detected inSKOV-3 cells, mouse spleen tissue
Positive IP detected inmouse spleen tissue
Positive IHC detected inmouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells
Positive FC (Intra) detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17373-1-AP targets PADI4/PAD4 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP, CoIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat
免疫原 PADI4/PAD4 fusion protein Ag11068 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 peptidyl arginine deiminase, type IV
别名 PADI4, EC:3.5.3.15, HL 60 PAD, HL-60 PAD, PAD
计算分子量 663 aa, 74 kDa
观测分子量 67 kDa
GenBank蛋白编号BC025718
基因名称 PADI4
Gene ID (NCBI) 23569
RRIDAB_2878398
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UM07
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PADI4, also named PADI5 and PDI5, catalyzes the citrullination/deimination of arginine residues of proteins such as histones, thereby playing a key role in histone code and regulation of stem cell maintenance. PADI4 is expressed in hematologic and rheumatoid arthritis synovial tissues. PADI4 haplotype associated with susceptibility to rheumatoid arthritis increases the production of citrullinated peptides, leading, in a cytokine-rich milieu, to a break in tolerance to citrullinated peptides processed and presented in the appropriate HLA context. Immunohistochemistry detected significant PADI4 expression in various malignancies and PADI4 expression was not detected in non-tumor tissues. (PMID: 19183436)

实验方案

Product Specific Protocols
WB protocol for PADI4/PAD4 antibody 17373-1-APDownload protocol
IHC protocol for PADI4/PAD4 antibody 17373-1-APDownload protocol
IF protocol for PADI4/PAD4 antibody 17373-1-APDownload protocol
IP protocol for PADI4/PAD4 antibody 17373-1-APDownload protocol
FC protocol for PADI4/PAD4 antibody 17373-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Int J Mol Sci

Investigation of H2S Donor Treatment on Neutrophil Extracellular Traps in Experimental Colitis.

Authors - Szilvia Török
mouseWB

RMD Open

IL-6-PAD4 axis in the earliest phase of arthritis in knock-in gp130F759 mice, a model for rheumatoid arthritis.

Authors - Ayano Yahagi
humanWB, IF

Transl Stroke Res

Neutrophil Extracellular Traps may be a Potential Target for Treating Early Brain Injury in Subarachnoid Hemorrhage.

Authors - Hanhai Zeng
humanWB

Int Immunopharmacol

IDH1/MDH1 deacetylation promotes NETosis by regulating OPA1 and autophagy

Authors - Yukun Wang
ratIF

Int J Mol Sci

Loss of 15-Lipoxygenase in Retinodegenerative RCS Rats

Authors - Andrew James Mead
mouseWB

Virulence

Extracellular trap can be trained as a memory response.

Authors - Yu Gao
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