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  • Featured Product
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PADI4 Polyclonal antibody

PADI4 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 17373-1-AP

Print datasheet

Synonyms

PADI5, PAD4, PAD, HL-60 PAD, HL 60 PAD

验证数据展示

  • WB analysis of SKOV-3 using 17373-1-AP

    WB analysis of SKOV-3 using 17373-1-AP

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 17373-1-AP (PADI4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse spleen using 17373-1-AP

    IHC staining of mouse spleen using 17373-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 17373-1-AP (PADI4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A549 using 17373-1-AP

    IF Staining of A549 using 17373-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using PADI4 antibody (17373-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • FC experiment of HeLa using 17373-1-AP

    FC experiment of HeLa using 17373-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human PADI4 (17373-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IHC staining of mouse spleen using 17373-1-AP

    IHC staining of mouse spleen using 17373-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 17373-1-AP (PADI4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of mouse spleen using 17373-1-AP

    WB analysis of mouse spleen using 17373-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 17373-1-AP (PADI4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse spleen using 17373-1-AP

    IP experiment of mouse spleen using 17373-1-AP

    IP result of anti-PADI4 (IP:17373-1-AP, 4ug; Detection:17373-1-AP 1:500) with mouse spleen tissue lysate 4800ug.

  • IF Staining of A549 using 17373-1-AP

    IF Staining of A549 using 17373-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using PADI4 antibody (17373-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inSKOV-3 cells, mouse spleen tissue
Positive IP detected inmouse spleen tissue
Positive IHC detected inmouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells
Positive FC (Intra) detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17373-1-AP targets PADI4 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP, CoIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen PADI4 fusion protein Ag11068 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name peptidyl arginine deiminase, type IV
Synonyms PADI5, PAD4, PAD, HL-60 PAD, HL 60 PAD
Calculated Molecular Weight 663 aa, 74 kDa
Observed Molecular Weight 67 kDa
GenBank Accession NumberBC025718
Gene Symbol PADI4
Gene ID (NCBI) 23569
RRIDAB_2878398
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UM07
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PADI4, also named PADI5 and PDI5, catalyzes the citrullination/deimination of arginine residues of proteins such as histones, thereby playing a key role in histone code and regulation of stem cell maintenance. PADI4 is expressed in hematologic and rheumatoid arthritis synovial tissues. PADI4 haplotype associated with susceptibility to rheumatoid arthritis increases the production of citrullinated peptides, leading, in a cytokine-rich milieu, to a break in tolerance to citrullinated peptides processed and presented in the appropriate HLA context. Immunohistochemistry detected significant PADI4 expression in various malignancies and PADI4 expression was not detected in non-tumor tissues. (PMID: 19183436)

实验方案

Product Specific Protocols
WB protocol for PADI4 antibody 17373-1-APDownload protocol
IHC protocol for PADI4 antibody 17373-1-APDownload protocol
IF protocol for PADI4 antibody 17373-1-APDownload protocol
IP protocol for PADI4 antibody 17373-1-APDownload protocol
FC protocol for PADI4 antibody 17373-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Int J Mol Sci

Investigation of H2S Donor Treatment on Neutrophil Extracellular Traps in Experimental Colitis.

Authors - Szilvia Török
View Article
mouseWB

RMD Open

IL-6-PAD4 axis in the earliest phase of arthritis in knock-in gp130F759 mice, a model for rheumatoid arthritis.

Authors - Ayano Yahagi
View Article
humanWB, IF

Transl Stroke Res

Neutrophil Extracellular Traps may be a Potential Target for Treating Early Brain Injury in Subarachnoid Hemorrhage.

Authors - Hanhai Zeng
View Article
ratIF

Int J Mol Sci

Loss of 15-Lipoxygenase in Retinodegenerative RCS Rats

Authors - Andrew James Mead
View Article
humanWB

Int Immunopharmacol

IDH1/MDH1 deacetylation promotes NETosis by regulating OPA1 and autophagy

Authors - Yukun Wang
View Article
mouseWB

Virulence

Extracellular trap can be trained as a memory response.

Authors - Yu Gao
View Article