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验证数据展示

  • IF Staining of A549 using CL488-17373

    IF Staining of A549 using CL488-17373

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using CoraLite® Plus 488 PADI4 antibody (CL488-17373) at dilution of 1:200.

  • FC experiment of HeLa using CL488-17373

    FC experiment of HeLa using CL488-17373

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human PADI4 (CL488-17373) (red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated PADI4/PAD4 Polyclonal antibody

PADI4/PAD4 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-17373

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

PADI4, EC:3.5.3.15, HL 60 PAD, HL-60 PAD, PAD

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inA549 cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 17373-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-17373 targets PADI4/PAD4 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 PADI4/PAD4 fusion protein Ag11068 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 peptidyl arginine deiminase, type IV
别名 PADI4, EC:3.5.3.15, HL 60 PAD, HL-60 PAD, PAD
计算分子量 663 aa, 74 kDa
观测分子量 67 kDa
GenBank蛋白编号BC025718
基因名称 PADI4
Gene ID (NCBI) 23569
RRIDAB_3084004
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UM07
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PADI4, also named as PADI5 and PDI5, catalyzes the citrullination/deimination of arginine residues of proteins such as histones, thereby playing a key role in histone code and regulation of stem cell maintenance. PADI4 is expressed in hematologic and rheumatoid arthritis synovial tissues. PADI4 haplotype associated with susceptibility to rheumatoid arthritis increases production of citrullinated peptides, leading, in a cytokine-rich milieu, to a break in tolerance to citrullinated peptides processed and presented in the appropriate HLA context. Immunohistochemistry detected significant PADI4 expression in various malignancies and PADI4 expression was not detected in non-tumor tissues.(PMID: 19183436)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 PADI4/PAD4 antibody CL488-17373Download protocol
Standard Protocols
Click here to view our Standard Protocols