验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human ovary tumor tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 6 publications below |
产品信息
15441-1-AP targets PAF1 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | PAF1 fusion protein Ag7212 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | Paf1, RNA polymerase II associated factor, homolog (S. cerevisiae) |
| 别名 | F23149_1, hPAF1, PAF1, PD2 |
| 计算分子量 | 60 kDa |
| 观测分子量 | 80 kDa |
| GenBank蛋白编号 | BC000017 |
| 基因名称 | PAF1 |
| Gene ID (NCBI) | 54623 |
| RRID | AB_2174457 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8N7H5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PAF1, also known as hPAF1 or PD2, is a PAF (RNA Polymerase II Associated Factor) complex subunit. Paf1 functions primarily to modulates transcript elongation. PAF1 occupies transcriptional enhancers and restrains hyperactivation of a subset of these enhancers(PMID: 28860207). The PAF complex also has a role in histone monoubiquitination. Paf1 is overexpressed in pancreatic cancer cells and has demonstrated potential oncogenic property. The MW of this protein is 80 kDa, and this antibody specially recognises the 80 kDa protein.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PAF1 antibody 15441-1-AP | Download protocol |
| IHC protocol for PAF1 antibody 15441-1-AP | Download protocol |
| IP protocol for PAF1 antibody 15441-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Autophagy The Paf1 complex transcriptionally regulates the mitochondrial-anchored protein Atg32 leading to activation of mitophagy.
| ||
J Cell Biol Phase separation and toxicity of C9orf72 poly(PR) depends on alternate distribution of arginine. | ||
Radiat Oncol Novel role of PAF1 in attenuating radiosensitivity in cervical cancer by inhibiting IER5 transcription. | ||
ACS Pharmacol Transl Sci Identification of PDCD2 as a Candidate Target of Andrographolide That Arrests the Tumor Cell Cycle by Human Proteome-Scale Screening | ||
Sci Adv CDK12 and Integrator-PP2A complex modulates LEO1 phosphorylation for processive transcription elongation | ||
