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验证数据展示

  • IF Staining of HepG2 using CL594-66261

    IF Staining of HepG2 using CL594-66261

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594-conjugated PAI-1 antibody (CL594-66261, Clone: 1H4A5 ) at dilution of 1:100.

CoraLite®594-conjugated Serpin E1/PAI-1 Monoclonal antibody

Serpin E1/PAI-1 Monoclonal Antibody for IF/ICC
Cat No. CL594-66261

产品说明书

CloneNo. 1H4A5

宿主/亚型

Mouse / IgG1

种属反应性

human, pig

应用

IF/ICC

别名

PAI-1, SERPINE1, SERPINE1, PAI-1, Plasminogen activator inhibitor 1, PAI1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy SERPINE1 Ready-To-Use IHC Kit. SERPINE1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66261-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66261 targets Serpin E1/PAI-1 in IF/ICC applications and shows reactivity with human, pig samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, pig
免疫原 Serpin E1/PAI-1 fusion protein Ag22411 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1
别名 PAI-1, SERPINE1, SERPINE1, PAI-1, Plasminogen activator inhibitor 1, PAI1
计算分子量 402 aa, 45 kDa
观测分子量 50 kDa
GenBank蛋白编号BC010860
基因名称 PAI-1
Gene ID (NCBI) 5054
RRIDAB_2883545
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP05121
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SERPINE1, also named as Plasminogen activator inhibitor type 1 (PAI-1), is a member of the serine protease inhibitor (SERPIN) superfamily. PAI-1 is the principal inhibitor of tissue plasminogen activator (tPA) and uPA, and hence is an inhibitor of fibrinolysis. Defects in this gene are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency), and high concentrations of the gene product are associated with thrombophilia.

实验方案

Product Specific Protocols
IF protocol for CL594 Serpin E1/PAI-1 antibody CL594-66261Download protocol
Standard Protocols
Click here to view our Standard Protocols