• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 68042-1-Ig

    WB analysis using 68042-1-Ig

    A549 cells were subjected to SDS PAGE followed by western blot with 68042-1-Ig (PAIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68042-1-Ig

    WB analysis using 68042-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68042-1-Ig (PAIP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IHC staining of human liver cancer using 68042-1-Ig

    IHC staining of human liver cancer using 68042-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68042-1-Ig (PAIP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PAIP1 Monoclonal antibody

PAIP1 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 68042-1-Ig

产品说明书

CloneNo. 2H9D4

宿主/亚型

Mouse / IgG2b

种属反应性

Human, mouse, rabbit

应用

WB, IHC, ELISA

别名

PABP interacting protein 1, PAIP 1, PAIP1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inmouse brain tissue, A549 cells, rabbit brain tissue, LNCaP cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68042-1-Ig targets PAIP1 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rabbit samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, mouse, rabbit
免疫原 PAIP1 fusion protein Ag16773 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 poly(A) binding protein interacting protein 1
别名 PABP interacting protein 1, PAIP 1, PAIP1
计算分子量 70 kDa
观测分子量40-45kDa, 62kDa
GenBank蛋白编号BC005295
基因名称 PAIP1
Gene ID (NCBI) 10605
RRIDAB_2918784
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9H074
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PAIP1 is a PABP-binding protein that contains two distinct PABP-binding motifs (PAMs). PAM1 binds to RNA recognition motif 2 in the N terminus of PABP; PAM2, which is a conserved region comprising approximately 15 amino acids, binds to the PABC domain of PABP. PAIP1 acts as a coactivator in the regulation of translation initiation of poly(A)-containing mRNAs.

实验方案

Product Specific Protocols
WB protocol for PAIP1 antibody 68042-1-IgDownload protocol
IHC protocol for PAIP1 antibody 68042-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols