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PAIP1 Monoclonal antibody

PAIP1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rabbit

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2H9D4

Cat no : 68042-1-Ig

Print datasheet

Synonyms

PABP interacting protein 1, PAIP 1, PAIP1

验证数据展示

  • WB analysis using 68042-1-Ig

    WB analysis using 68042-1-Ig

    A549 cells were subjected to SDS PAGE followed by western blot with 68042-1-Ig (PAIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68042-1-Ig

    WB analysis using 68042-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68042-1-Ig (PAIP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IHC staining of human liver cancer using 68042-1-Ig

    IHC staining of human liver cancer using 68042-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68042-1-Ig (PAIP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse brain tissue, A549 cells, rabbit brain tissue, LNCaP cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68042-1-Ig targets PAIP1 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rabbit samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human, mouse, rabbit
Immunogen PAIP1 fusion protein Ag16773 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name poly(A) binding protein interacting protein 1
Synonyms PABP interacting protein 1, PAIP 1, PAIP1
Calculated Molecular Weight 70 kDa
Observed Molecular Weight40-45kDa, 62kDa
GenBank Accession NumberBC005295
Gene Symbol PAIP1
Gene ID (NCBI) 10605
RRIDAB_2918784
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9H074
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PAIP1 is a PABP-binding protein that contains two distinct PABP-binding motifs (PAMs). PAM1 binds to RNA recognition motif 2 in the N terminus of PABP; PAM2, which is a conserved region comprising approximately 15 amino acids, binds to the PABC domain of PABP. PAIP1 acts as a coactivator in the regulation of translation initiation of poly(A)-containing mRNAs.

实验方案

Product Specific Protocols
WB protocol for PAIP1 antibody 68042-1-IgDownload protocol
IHC protocol for PAIP1 antibody 68042-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols