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PAIP1 Monoclonal antibody, PBS Only

PAIP1 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rabbit

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2H9D4

Cat no : 68042-1-PBS

Print datasheet

Synonyms

PABP interacting protein 1, PAIP 1, PAIP1

验证数据展示

  • WB analysis using 68042-1-Ig (same clone as 68042-1-PBS)

    WB analysis using 68042-1-Ig (same clone as 68042-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 68042-1-Ig (PAIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68042-1-PBS in a different storage buffer formulation.

  • WB analysis using 68042-1-Ig (same clone as 68042-1-PBS)

    WB analysis using 68042-1-Ig (same clone as 68042-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68042-1-Ig (PAIP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68042-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 68042-1-Ig (same clone as 68042-1-PBS)

    IHC staining of human liver cancer using 68042-1-Ig (same clone as 68042-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68042-1-Ig (PAIP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68042-1-PBS in a different storage buffer formulation.

产品信息

68042-1-PBS targets PAIP1 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rabbit samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity Human, mouse, rabbit
Immunogen PAIP1 fusion protein Ag16773 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name poly(A) binding protein interacting protein 1
Synonyms PABP interacting protein 1, PAIP 1, PAIP1
Calculated Molecular Weight 70 kDa
Observed Molecular Weight40-45kDa, 62kDa
GenBank Accession NumberBC005295
Gene Symbol PAIP1
Gene ID (NCBI) 10605
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9H074
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PAIP1 is a PABP-binding protein that contains two distinct PABP-binding motifs (PAMs). PAM1 binds to RNA recognition motif 2 in the N terminus of PABP; PAM2, which is a conserved region comprising approximately 15 amino acids, binds to the PABC domain of PABP. PAIP1 acts as a coactivator in the regulation of translation initiation of poly(A)-containing mRNAs.