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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 68298-1-Ig (same clone as 68298-1-PBS)

    WB analysis using 68298-1-Ig (same clone as 68298-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68298-1-Ig (PAK4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68298-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 68298-1-Ig (same clone as 68298-1-PBS)

    IF Staining of HeLa using 68298-1-Ig (same clone as 68298-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PAK4 antibody (68298-1-Ig, Clone: 2G6A3 ) at dilution of 1:3000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68298-1-PBS in a different storage buffer formulation.

PAK4 Monoclonal antibody, PBS Only

PAK4 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 68298-1-PBS

产品说明书

CloneNo. 2G6A3

宿主/亚型

Mouse / IgG2b

种属反应性

Human, Mouse, Rat

应用

WB, IF/ICC, Indirect ELISA

别名

KIAA1142, p21 activated kinase 4, PAK 4, PAK4

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

68298-1-PBS targets PAK4 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 Human, Mouse, Rat
免疫原 PAK4 fusion protein Ag6364 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 p21 protein (Cdc42/Rac)-activated kinase 4
别名 KIAA1142, p21 activated kinase 4, PAK 4, PAK4
计算分子量 64 kDa
观测分子量64 kDa
GenBank蛋白编号BC025282
基因名称 PAK4
Gene ID (NCBI) 10298
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO96013
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PAK4(p21-activated kinase 4) is also named as KIAA1142. It belongs to the protein kinase superfamily.PAK4 regulates cell morphology, cytoskeletal organization, and cell proliferation and migration. It can also function as an antiapoptotic protein (PMID:14517283). It has 4 isoforms produced by alternative splicing. And it can be autophosphorylated on serine residues when activated by CDC42/p21 (PMID:11278822).