验证数据展示
经过测试的应用
Positive WB detected in | A549 cells, MCF-7 cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells |
Positive IF/ICC detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:1500-1:6000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
68298-1-Ig targets PAK4 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
Tested Applications | WB, IF/ICC, ELISA Application Description |
Tested Reactivity | Human, Mouse, Rat |
Immunogen | PAK4 fusion protein Ag6364 种属同源性预测 |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Full Name | p21 protein (Cdc42/Rac)-activated kinase 4 |
Synonyms | KIAA1142, p21 activated kinase 4, PAK 4, PAK4 |
Calculated Molecular Weight | 64 kDa |
Observed Molecular Weight | 64 kDa |
GenBank Accession Number | BC025282 |
Gene Symbol | PAK4 |
Gene ID (NCBI) | 10298 |
RRID | AB_2935378 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | O96013 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PAK4(p21-activated kinase 4) is also named as KIAA1142. It belongs to the protein kinase superfamily.PAK4 regulates cell morphology, cytoskeletal organization, and cell proliferation and migration. It can also function as an antiapoptotic protein (PMID:14517283). It has 4 isoforms produced by alternative splicing. And it can be autophosphorylated on serine residues when activated by CDC42/p21 (PMID:11278822).
实验方案
Product Specific Protocols | |
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WB protocol for PAK4 antibody 68298-1-Ig | Download protocol |
IF protocol for PAK4 antibody 68298-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |