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验证数据展示

  • IF Staining of HeLa using CL488-68298

    IF Staining of HeLa using CL488-68298

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 PAK4 antibody (CL488-68298, Clone: 2G6A3 ) at dilution of 1:100.

CoraLite® Plus 488-conjugated PAK4 Monoclonal antibody

PAK4 Monoclonal Antibody for IF/ICC
Cat No. CL488-68298
CloneNo. 2G6A3

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat

Applications

IF/ICC

Synonyms

KIAA1142, p21 activated kinase 4, PAK 4, PAK4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488

Size: 
100 μL

¥2800/ 100 μL

浓度: 1000 μg/ml

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经过测试的应用

Positive IF/ICC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 68298-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68298 targets PAK4 in IF/ICC applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen PAK4 fusion protein Ag6364 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name p21 protein (Cdc42/Rac)-activated kinase 4
Synonyms KIAA1142, p21 activated kinase 4, PAK 4, PAK4
Calculated Molecular Weight 64 kDa
Observed Molecular Weight64 kDa
GenBank Accession NumberBC025282
Gene Symbol PAK4
Gene ID (NCBI) 10298
RRIDAB_3672991
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO96013
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PAK4(p21-activated kinase 4) is also named as KIAA1142. It belongs to the protein kinase superfamily.PAK4 regulates cell morphology, cytoskeletal organization, and cell proliferation and migration. It can also function as an antiapoptotic protein (PMID:14517283). It has 4 isoforms produced by alternative splicing. And it can be autophosphorylated on serine residues when activated by CDC42/p21 (PMID:11278822).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 PAK4 antibody CL488-68298Download protocol
Standard Protocols
Click here to view our Standard Protocols