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验证数据展示

  • WB analysis of K-562 using 67562-1-Ig

    WB analysis of K-562 using 67562-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 67562-1-Ig (PAK6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 67562-1-Ig

    WB analysis of HEK-293 using 67562-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67562-1-Ig (PAK6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293 using 67562-1-Ig

    IF Staining of HEK-293 using 67562-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using PAK6 antibody (67562-1-Ig, Clone: 3G8C1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

PAK6 Monoclonal antibody

PAK6 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67562-1-Ig

产品说明书

CloneNo. 3G8C1

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

WB, IF/ICC, ELISA

别名

p21 activated kinase 6, PAK 5, PAK 6, PAK5, PAK6

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, K-562 cells
Positive IF/ICC detected inHEK-293 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67562-1-Ig targets PAK6 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human
免疫原 PAK6 fusion protein Ag27692 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 p21 protein (Cdc42/Rac)-activated kinase 6
别名 p21 activated kinase 6, PAK 5, PAK 6, PAK5, PAK6
计算分子量 681 aa, 75 kDa
观测分子量70-75 kDa
GenBank蛋白编号BC035596
基因名称 PAK6
Gene ID (NCBI) 56924
RRIDAB_2882776
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9NQU5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

PAK6(p21-activated kinase 6) is a serine/threonine kinase belonging to the p21-activated kinase (PAK) family. .This protein plays a role in the regulation of gene transcription and may protect cells from apoptosis through phosphorylation of BAD. Northern blot analysis revealed that PAK6 is highly expressed in testis and prostate tissues (PMID:11278661).

实验方案

Product Specific Protocols
WB protocol for PAK6 antibody 67562-1-IgDownload protocol
IF protocol for PAK6 antibody 67562-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols