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验证数据展示

  • WB analysis of K-562 using 67562-1-Ig

    WB analysis of K-562 using 67562-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 67562-1-Ig (PAK6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 67562-1-Ig

    WB analysis of HEK-293 using 67562-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67562-1-Ig (PAK6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293 using 67562-1-Ig

    IF Staining of HEK-293 using 67562-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using PAK6 antibody (67562-1-Ig, Clone: 3G8C1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

PAK6 Monoclonal antibody

PAK6 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67562-1-Ig

Print datasheet

CloneNo. 3G8C1

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Synonyms

p21 activated kinase 6, PAK 5, PAK 6, PAK5, PAK6

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, K-562 cells
Positive IF/ICC detected inHEK-293 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67562-1-Ig targets PAK6 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity Human
Immunogen PAK6 fusion protein Ag27692 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name p21 protein (Cdc42/Rac)-activated kinase 6
Synonyms p21 activated kinase 6, PAK 5, PAK 6, PAK5, PAK6
Calculated Molecular Weight 681 aa, 75 kDa
Observed Molecular Weight70-75 kDa
GenBank Accession NumberBC035596
Gene Symbol PAK6
Gene ID (NCBI) 56924
RRIDAB_2882776
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NQU5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

PAK6(p21-activated kinase 6) is a serine/threonine kinase belonging to the p21-activated kinase (PAK) family. .This protein plays a role in the regulation of gene transcription and may protect cells from apoptosis through phosphorylation of BAD. Northern blot analysis revealed that PAK6 is highly expressed in testis and prostate tissues (PMID:11278661).

实验方案

Product Specific Protocols
WB protocol for PAK6 antibody 67562-1-IgDownload protocol
IF protocol for PAK6 antibody 67562-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols