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验证数据展示

  • IF Staining of HEK-293 using CL488-67562

    IF Staining of HEK-293 using CL488-67562

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CoraLite® Plus 488 PAK6 antibody (CL488-67562, Clone: 3G8C1 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated PAK6 Monoclonal antibody

PAK6 Monoclonal Antibody for IF/ICC
Cat No. CL488-67562

Print datasheet

CloneNo. 3G8C1

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF/ICC

Synonyms

p21 activated kinase 6, PAK 5, PAK 6, PAK5, PAK6

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHEK-293 cells
For other applications, we recommend the unconjugated version of this antibody, 67562-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67562 targets PAK6 in IF/ICC applications and shows reactivity with Human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human
Immunogen PAK6 fusion protein Ag27692 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name p21 protein (Cdc42/Rac)-activated kinase 6
Synonyms p21 activated kinase 6, PAK 5, PAK 6, PAK5, PAK6
Calculated Molecular Weight 681 aa, 75 kDa
Observed Molecular Weight70-75 kDa
GenBank Accession NumberBC035596
Gene Symbol PAK6
Gene ID (NCBI) 56924
RRIDAB_3084357
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9NQU5
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PAK6(p21-activated kinase 6) is a serine/threonine kinase belonging to the p21-activated kinase (PAK) family. .This protein plays a role in the regulation of gene transcription and may protect cells from apoptosis through phosphorylation of BAD. Northern blot analysis revealed that PAK6 is highly expressed in testis and prostate tissues (PMID:11278661).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 PAK6 antibody CL488-67562Download protocol
Standard Protocols
Click here to view our Standard Protocols