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PARD3 Monoclonal antibody, PBS Only

PARD3 Monoclonal Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D2B2

Cat no : 66968-1-PBS

Print datasheet

Synonyms

ASIP, Baz, Bazooka, CTCL tumor antigen se2 5, FLJ21015, PAR 3, PAR3, PAR3 alpha, PAR3A, PAR3alpha, PARD 3, PARD3, PARD3A, SE2 5L16, SE2 5LT1, SE2 5T2

验证数据展示

  • WB analysis of MCF-7 using 66968-1-Ig (same clone as 66968-1-PBS)

    WB analysis of MCF-7 using 66968-1-Ig (same clone as 66968-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 66968-1-Ig (PARD3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66968-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 66968-1-Ig (same clone as 66968-1-PBS)

    IF Staining of MCF-7 using 66968-1-Ig (same clone as 66968-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using PARD3 antibody (66968-1-Ig, Clone: 1D2B2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66968-1-PBS in a different storage buffer formulation.

  • FC experiment of MCF-7 using 66968-1-Ig (same clone as 66968-1-PBS)

    FC experiment of MCF-7 using 66968-1-Ig (same clone as 66968-1-PBS)

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human PARD3 (66968-1-Ig, Clone:1D2B2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66968-1-PBS in a different storage buffer formulation.

产品信息

66968-1-PBS targets PARD3 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IF/ICC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity Human
Immunogen PARD3 fusion protein Ag7656 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name par-3 partitioning defective 3 homolog (C. elegans)
Synonyms ASIP, Baz, Bazooka, CTCL tumor antigen se2 5, FLJ21015, PAR 3, PAR3, PAR3 alpha, PAR3A, PAR3alpha, PARD 3, PARD3, PARD3A, SE2 5L16, SE2 5LT1, SE2 5T2
Calculated Molecular Weight 151 kDa
Observed Molecular Weight 170 kDa
GenBank Accession NumberBC011711
Gene Symbol PARD3
Gene ID (NCBI) 56288
RRIDAB_2882290
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ8TEW0
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PARD3 (also known as ASIP, Par3, or Bazooka) is one of PARD proteins which are essential for asymmetric cell division and polarized growth. PARD3 is involved in the establishment of cell polarity and in the asymmetric cytokinesis. It plays a role in tight junctions at epithelial cell-cell contacts.