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PARD3 Monoclonal antibody

PARD3 Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1D2B2

Cat no : 66968-1-Ig

Print datasheet

Synonyms

ASIP, Baz, Bazooka, CTCL tumor antigen se2 5, FLJ21015, PAR 3, PAR3, PAR3 alpha, PAR3A, PAR3alpha, PARD 3, PARD3, PARD3A, SE2 5L16, SE2 5LT1, SE2 5T2

验证数据展示

  • WB analysis of MCF-7 using 66968-1-Ig

    WB analysis of MCF-7 using 66968-1-Ig

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 66968-1-Ig (PARD3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 66968-1-Ig

    IF Staining of MCF-7 using 66968-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using PARD3 antibody (66968-1-Ig, Clone: 1D2B2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • FC experiment of MCF-7 using 66968-1-Ig

    FC experiment of MCF-7 using 66968-1-Ig

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human PARD3 (66968-1-Ig, Clone:1D2B2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inMCF-7 cells, HeLa cells, HEK-293 cells, A431 cells, HepG2 cells
Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inMCF-7 cells
Positive FC detected inMCF-7 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66968-1-Ig targets PARD3 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity Human
Immunogen PARD3 fusion protein Ag7656 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name par-3 partitioning defective 3 homolog (C. elegans)
Synonyms ASIP, Baz, Bazooka, CTCL tumor antigen se2 5, FLJ21015, PAR 3, PAR3, PAR3 alpha, PAR3A, PAR3alpha, PARD 3, PARD3, PARD3A, SE2 5L16, SE2 5LT1, SE2 5T2
Calculated Molecular Weight 151 kDa
Observed Molecular Weight 170 kDa
GenBank Accession NumberBC011711
Gene Symbol PARD3
Gene ID (NCBI) 56288
RRIDAB_2882290
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ8TEW0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PARD3 (also known as ASIP, Par3, or Bazooka) is one of PARD proteins which are essential for asymmetric cell division and polarized growth. PARD3 is involved in the establishment of cell polarity and in the asymmetric cytokinesis. It plays a role in tight junctions at epithelial cell-cell contacts.

实验方案

Product Specific Protocols
WB protocol for PARD3 antibody 66968-1-IgDownload protocol
IF protocol for PARD3 antibody 66968-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols