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CoraLite® Plus 488-conjugated PARD3 Monoclonal antibody

PARD3 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1D2B2

Cat no : CL488-66968

Print datasheet

Synonyms

ASIP, Baz, Bazooka, CTCL tumor antigen se2 5, FLJ21015, PAR 3, PAR3, PAR3 alpha, PAR3A, PAR3alpha, PARD 3, PARD3, PARD3A, SE2 5L16, SE2 5LT1, SE2 5T2

验证数据展示

  • IF Staining of MCF-7 using CL488-66968

    IF Staining of MCF-7 using CL488-66968

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite® Plus 488 PARD3 antibody (CL488-66968, Clone: 1D2B2 ) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inMCF-7 cells
For other applications, we recommend the unconjugated version of this antibody, 66968-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66968 targets PARD3 in IF/ICC applications and shows reactivity with Human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human
Immunogen PARD3 fusion protein Ag7656 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name par-3 partitioning defective 3 homolog (C. elegans)
Synonyms ASIP, Baz, Bazooka, CTCL tumor antigen se2 5, FLJ21015, PAR 3, PAR3, PAR3 alpha, PAR3A, PAR3alpha, PARD 3, PARD3, PARD3A, SE2 5L16, SE2 5LT1, SE2 5T2
Calculated Molecular Weight 151 kDa
Observed Molecular Weight 170 kDa
GenBank Accession NumberBC011711
Gene Symbol PARD3
Gene ID (NCBI) 56288
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ8TEW0
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PARD3 (also known as ASIP, Par3, or Bazooka) is one of PARD proteins which are essential for asymmetric cell division and polarized growth. PARD3 is involved in the establishment of cell polarity and in the asymmetric cytokinesis. It plays a role in tight junctions at epithelial cell-cell contacts.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 PARD3 antibody CL488-66968Download protocol
Standard Protocols
Click here to view our Standard Protocols