ChromoTek PARP1-Trap Agarose beads
PARP1-Trap Agarose is an affinity reagent for IP of human PARP1. It consists of a PARP1 Nanobody/ VHH, which is coupled to agarose beads.
Cat No : xta
Synonyms
Validation Data Gallery
Product Information
PARP1-Trap Agarose is an affinity reagent for IP of human PARP1. It consists of a PARP1 Nanobody/ VHH, which is coupled to agarose beads.
| Applications | IP, CoIP |
| Clonality | Monoclonal |
| Host | Alpaca |
| Specificity/Target | Human PARP1, does not bind to PARP2, 3, and 9 |
| Coupled VHH | Recombinant, monoclonal anti-PARP1 single domain antibody (sdAb) fragment |
| Conjugate | Agarose |
| Beads | ~ 90 µm (cross-linked 4 % agarose beads) |
| Elution buffer | SDS sample buffer 0.2 M glycine pH 2.5 |
| Storage Buffer | 20% EtOH |
| Storage Condition | Shipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. |
Publications
| Application | Title |
|---|---|
PLoS One A New Nanobody-Based Biosensor to Study Endogenous PARP1 In Vitro and in Live Human Cells. | |
EMBO Rep Ewing sarcoma protein promotes dissociation of poly(ADP-ribose) polymerase 1 from chromatin. | |
EMBO J Unanchored tri-NEDD8 inhibits PARP-1 to protect from oxidative stress-induced cell death. | |