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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 16110-1-AP (same clone as 16110-1-PBS)

    WB analysis using 16110-1-AP (same clone as 16110-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16110-1-AP (PBK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16110-1-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 16110-1-AP (same clone as 16110-1-PBS)

    IHC staining of human placenta using 16110-1-AP (same clone as 16110-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16110-1-AP (PBK antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16110-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PBK antibody included
  • IHC staining of human placenta using 16110-1-AP (same clone as 16110-1-PBS)

    IHC staining of human placenta using 16110-1-AP (same clone as 16110-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16110-1-AP (PBK antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16110-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PBK antibody included
  • WB analysis using 16110-1-AP (same clone as 16110-1-PBS)

    WB analysis using 16110-1-AP (same clone as 16110-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16110-1-AP (PBK antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16110-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 16110-1-AP (same clone as 16110-1-PBS)

    FC experiment of HepG2 using 16110-1-AP (same clone as 16110-1-PBS)

    (blue)1x10^6 HepG2 cells were intracellularly stained with 0.8 ug Anti-Human PBK (16110-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2) at dilution 1:1000(red), or 0.8 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 16110-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

PBK/SPK Polyclonal antibody, PBS Only

PBK/SPK Polyclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA
Cat No. 16110-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, FC (Intra), Indirect ELISA

别名

PBK, MAPKK-like protein kinase, MAPKK like protein kinase, Lymphokine-activated killer T-cell-originated protein kinase, EC:2.7.12.2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy PBK Ready-To-Use IHC Kit. PBK primary antibody included.

产品信息

16110-1-PBS targets PBK/SPK in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 PBK/SPK fusion protein Ag9033 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 PDZ binding kinase
别名 PBK, MAPKK-like protein kinase, MAPKK like protein kinase, Lymphokine-activated killer T-cell-originated protein kinase, EC:2.7.12.2
计算分子量 322 aa, 36 kDa
观测分子量 36-40 kDa
GenBank蛋白编号BC015191
基因名称 PBK
Gene ID (NCBI) 55872
RRIDAB_2283508
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96KB5
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PBK(PDZ-binding kinase) is also named as TOPK, CT84, Nori-3, SPK and belongs to the MAP kinase kinase subfamily. PBK may have a role in the regulation of cellular proliferation and progression of the cell cycle. It has a characteristic cdc2ycyclin B phosphorylation site(SyT-P-X-KyR) at its N terminus,which is conserved across species, and it is phosphorylated in a cell cycle-dependent manner at mitosis, and that this phosphorylation is required for its activation(PMID:10779557).