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  • Featured Product
  • KD/KO Validated

PBK/SPK Polyclonal antibody, PBS Only

PBK/SPK Polyclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

Cat no : 16110-1-PBS

Print datasheet

Synonyms

PBK, MAPKK-like protein kinase, MAPKK like protein kinase, Lymphokine-activated killer T-cell-originated protein kinase, EC:2.7.12.2

验证数据展示

  • WB analysis using 16110-1-AP (same clone as 16110-1-PBS)

    WB analysis using 16110-1-AP (same clone as 16110-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16110-1-AP (PBK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16110-1-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 16110-1-AP (same clone as 16110-1-PBS)

    IHC staining of human placenta using 16110-1-AP (same clone as 16110-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16110-1-AP (PBK antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16110-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PBK antibody included
  • IHC staining of human placenta using 16110-1-AP (same clone as 16110-1-PBS)

    IHC staining of human placenta using 16110-1-AP (same clone as 16110-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16110-1-AP (PBK antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16110-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PBK antibody included
  • WB analysis using 16110-1-AP (same clone as 16110-1-PBS)

    WB analysis using 16110-1-AP (same clone as 16110-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16110-1-AP (PBK antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16110-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 16110-1-AP (same clone as 16110-1-PBS)

    FC experiment of HepG2 using 16110-1-AP (same clone as 16110-1-PBS)

    (blue)1x10^6 HepG2 cells were intracellularly stained with 0.8 ug Anti-Human PBK (16110-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2) at dilution 1:1000(red), or 0.8 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 16110-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy PBK Ready-To-Use IHC Kit. PBK primary antibody included.

产品信息

16110-1-PBS targets PBK/SPK in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen PBK/SPK fusion protein Ag9033 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name PDZ binding kinase
Synonyms PBK, MAPKK-like protein kinase, MAPKK like protein kinase, Lymphokine-activated killer T-cell-originated protein kinase, EC:2.7.12.2
Calculated Molecular Weight 322 aa, 36 kDa
Observed Molecular Weight 36-40 kDa
GenBank Accession NumberBC015191
Gene Symbol PBK
Gene ID (NCBI) 55872
RRIDAB_2283508
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96KB5
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PBK(PDZ-binding kinase) is also named as TOPK, CT84, Nori-3, SPK and belongs to the MAP kinase kinase subfamily. PBK may have a role in the regulation of cellular proliferation and progression of the cell cycle. It has a characteristic cdc2ycyclin B phosphorylation site(SyT-P-X-KyR) at its N terminus,which is conserved across species, and it is phosphorylated in a cell cycle-dependent manner at mitosis, and that this phosphorylation is required for its activation(PMID:10779557).