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PBX1 Polyclonal antibody

PBX1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA and More (3)

Conjugate

Unconjugated

Cat no : 18204-1-AP

Print datasheet

Synonyms

PRL, Pre-B-cell leukemia transcription factor 1, pre B cell leukemia homeobox 1, Homeobox protein PRL, Homeobox protein PBX1

验证数据展示

  • WB analysis of A2780 using 18204-1-AP

    WB analysis of A2780 using 18204-1-AP

    A2780 cells were subjected to SDS PAGE followed by western blot with 18204-1-AP (PBX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of A2780 using 18204-1-AP

    IP experiment of A2780 using 18204-1-AP

    IP result of anti-PBX1 (IP:18204-1-AP, 4ug; Detection:18204-1-AP 1:500) with A2780 cells lysate 1000ug.

  • IF Staining of HeLa using 18204-1-AP

    IF Staining of HeLa using 18204-1-AP

    Immunofluorescent analysis of HeLa cells using 18204-1-AP (PBX1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of mouse kidney using 18204-1-AP

    IHC staining of mouse kidney using 18204-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 18204-1-AP (PBX1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PBX1 antibody included
  • IHC staining of human cervical cancer using 18204-1-AP

    IHC staining of human cervical cancer using 18204-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 18204-1-AP (PBX1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PBX1 antibody included
  • IHC staining of human thyroid cancer using 18204-1-AP

    IHC staining of human thyroid cancer using 18204-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 18204-1-AP (PBX1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PBX1 antibody included
  • IHC staining of human thyroid cancer using 18204-1-AP

    IHC staining of human thyroid cancer using 18204-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 18204-1-AP (PBX1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PBX1 antibody included
  • IHC staining of mouse brain using 18204-1-AP

    IHC staining of mouse brain using 18204-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18204-1-AP (PBX1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PBX1 antibody included
  • IHC staining of mouse pancreas using 18204-1-AP

    IHC staining of mouse pancreas using 18204-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 18204-1-AP (PBX1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PBX1 antibody included
  • IHC staining of rat brain using 18204-1-AP

    IHC staining of rat brain using 18204-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 18204-1-AP (PBX1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PBX1 antibody included
  • IHC staining of rat kidney using 18204-1-AP

    IHC staining of rat kidney using 18204-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 18204-1-AP (PBX1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PBX1 antibody included
  • IHC staining of rat pancreas using 18204-1-AP

    IHC staining of rat pancreas using 18204-1-AP

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 18204-1-AP (PBX1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PBX1 antibody included

经过测试的应用

Positive WB detected inA2780 cells, HeLa cells
Positive IP detected inA2780 cells
Positive IHC detected inhuman thyroid cancer tissue, human cervical cancer tissue, mouse brain tissue, mouse kidney tissue, mouse pancreas tissue, rat brain tissue, rat kidney tissue, rat pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PBX1 Ready-To-Use IHC Kit. PBX1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18204-1-AP targets PBX1 in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, CoIP, ChIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen PBX1 fusion protein Ag12817 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name pre-B-cell leukemia homeobox 1
Synonyms PRL, Pre-B-cell leukemia transcription factor 1, pre B cell leukemia homeobox 1, Homeobox protein PRL, Homeobox protein PBX1
Calculated Molecular Weight 430 aa, 47 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC101578
Gene Symbol PBX1
Gene ID (NCBI) 5087
RRIDAB_10951860
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP40424
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PBX1, also named as Pre-B-cell leukemia transcription factor 1, is a 430 amino acid protein, which belongs to the TALE/PBX homeobox family. PBX1 is expressed in all tissues except in cells of the B and T lineage. PBX1 acts as a transcriptional activator of PF4 in complex with MEIS1. PBX1 may have a role in steroidogenesis and, subsequently, sexual development and differentiation. Isoform PBX1b as part of a PDX1:PBX1b:MEIS2b complex in pancreatic acinar cells is involved in the transcriptional activation of the ELA1 enhancer. PBX1 exists two isoforms (PBX1a and PBX1b) and molecular weight of PBX1 is 47 kDa and 38 kDa.

实验方案

Product Specific Protocols
WB protocol for PBX1 antibody 18204-1-APDownload protocol
IHC protocol for PBX1 antibody 18204-1-APDownload protocol
IF protocol for PBX1 antibody 18204-1-APDownload protocol
IP protocol for PBX1 antibody 18204-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIHC

Nature

Divergent transcriptional regulation of astrocyte reactivity across disorders.

Authors - Joshua E Burda
View Article
human,ratWB,IF

Int J Oral Sci

Stromal thrombospondin 1 suppresses angiogenesis in oral submucous fibrosis

Authors - Xiao Yang
View Article
mouseWB

J Neurochem

Decreased levels of constitutive proteasomes in experimental autoimmune encephalomyelitis may be caused by a combination of subunit displacement and reduced Nfe2l1 expression.

Authors - Kara L Shanley
View Article
mouseWB

Neuroscience

Neuroprotective Action of Teriflunomide in a Mouse Model of Transient Middle Cerebral Artery Occlusion.

Authors - Zhengfang Lu
View Article
humanWB

Cell Death Dis

M6A-methylated circPOLR2B forms an R-loop and regulates the biological behavior of glioma stem cells through positive feedback loops

Authors - Hongda Lin
View Article
mouseChIP

Cell Mol Life Sci

Loss of PBX1 function in Leydig cells causes testicular dysgenesis and male sterility

Authors - Fei-Chen Wang
View Article