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验证数据展示

  • WB analysis using 84979-1-RR (same clone as 84979-1-PBS)

    WB analysis using 84979-1-RR (same clone as 84979-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84979-1-RR (PCM1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84979-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 84979-1-RR (same clone as 84979-1-PBS)

    IHC staining of human tonsillitis using 84979-1-RR (same clone as 84979-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84979-1-RR (PCM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84979-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PCM1 antibody included
  • IF Staining of HepG2 using 84979-1-RR (same clone as 84979-1-PBS)

    IF Staining of HepG2 using 84979-1-RR (same clone as 84979-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PCM1 antibody (84979-1-RR, Clone: 242199A9 ) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84979-1-PBS in a different storage buffer formulation.

  • FC experiment of K562 using 84979-1-RR (same clone as 84979-1-PBS)

    FC experiment of K562 using 84979-1-RR (same clone as 84979-1-PBS)

    1x10^6 K562 were intracellularly stained with 0.25 ug PCM1 Recombinant antibody (84979-1-RR, Clone:242199A9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84979-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84979-1-RR

    Affinity and Kinetic Characterization of 84979-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84979-1-RR against Human PCM1 were performed. The affinity constant is 21.0 nM.

PCM1 Recombinant antibody, PBS Only

PCM1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 84979-1-PBS

产品说明书

CloneNo. 242199A9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

pericentriolar material 1, PCM-1, PCM 1, hPCM-1, hPCM 1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy PCM1 Ready-To-Use IHC Kit. PCM1 primary antibody included.

产品信息

84979-1-PBS targets PCM1 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 PCM1 fusion protein Ag13977 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 pericentriolar material 1
别名 pericentriolar material 1, PCM-1, PCM 1, hPCM-1, hPCM 1
计算分子量 2024 aa, 228 kDa
观测分子量245-320 kDa
GenBank蛋白编号BC000453
基因名称 PCM1
Gene ID (NCBI) 5108
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ15154
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Pericentriolar material 1 (PCM1) is a component of centriolar satellites, which are electron dense granules scattered around centrosomes (PMID: 10579718). PCM1 is required for centrosome assembly and function. It acts as a scaffold to target several proteins to the centrosome in a dynein motor-dependent manner and regulate microtubular dynamics (PMID:18762586; 12403812). It is also involved in the biogenesis of cilia (PMID: 18772192). Chromosomal aberrations involving PCM1 gene are associated with papillary thyroid carcinomas and a variety of hematological malignancies, including atypical chronic myeloid leukemia and T-cell lymphoma. (PMID: 10980597; 16091753; 15805263; 16424865).