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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 647-conjugated PCM1 Polyclonal antibody

PCM1 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat no : CL647-19856

Print datasheet

Synonyms

PTC4, pericentriolar material 1, PCM-1, PCM 1, hPCM-1

验证数据展示

  • IF Staining of HeLa using CL647-19856

    IF Staining of HeLa using CL647-19856

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 647 PCM1 antibody (CL647-19856) at dilution of 1:100.

  • FC experiment of HeLa using CL647-19856

    FC experiment of HeLa using CL647-19856

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human PCM1 (CL647-19856) (red), or 0.2 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 19856-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-19856 targets PCM1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse
Immunogen PCM1 fusion protein Ag13929 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name pericentriolar material 1
Synonyms PTC4, pericentriolar material 1, PCM-1, PCM 1, hPCM-1
Calculated Molecular Weight 2024 aa, 228 kDa
Observed Molecular Weight 260-280 kDa
GenBank Accession NumberBC000453
Gene Symbol PCM1
Gene ID (NCBI) 5108
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15154
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Pericentriolar material 1 (PCM1) is a component of centriolar satellites, which are electron dense granules scattered around centrosomes (PMID: 10579718). PCM1 is required for centrosome assembly and function. It acts as a scaffold to target several proteins to the centrosome in a dynein motor-dependent manner and regulate microtubular dynamics (PMID:18762586; 12403812). It is also involved in the biogenesis of cilia (PMID: 18772192). Chromosomal aberrations involving PCM1 gene are associated with papillary thyroid carcinomas and a variety of hematological malignancies, including atypical chronic myeloid leukemia and T-cell lymphoma. (PMID: 10980597; 16091753; 15805263; 16424865).

实验方案

Product Specific Protocols
IF protocol for CL Plus 647 PCM1 antibody CL647-19856Download protocol
FC protocol for CL Plus 647 PCM1 antibody CL647-19856Download protocol
Standard Protocols
Click here to view our Standard Protocols