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验证数据展示

  • WB analysis using 60172-1-Ig

    WB analysis using 60172-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60172-1-Ig (PCMT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 60172-1-Ig

    IF Staining of HeLa using 60172-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PCMT1 antibody (60172-1-Ig, Clone: 3C8G8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

PCMT1 Monoclonal antibody

PCMT1 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 60172-1-Ig

产品说明书

CloneNo. 3C8G8

宿主/亚型

Mouse / IgG1

种属反应性

human, pig

应用

WB, IF/ICC, ELISA

别名

PCMT1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, Raji cells, pig brain tissue, HEK-293 cells, HepG2 cells, K-562 cells
Positive IF/ICC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60172-1-Ig targets PCMT1 in WB, IF/ICC, ELISA applications and shows reactivity with human, pig samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, pig
免疫原 PCMT1 fusion protein Ag6277 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 protein-L-isoaspartate (D-aspartate) O-methyltransferase
别名 PCMT1
计算分子量 25 kDa
观测分子量 25-27 kDa
GenBank蛋白编号BC008748
基因名称 PCMT1
Gene ID (NCBI) 5110
RRIDAB_10695773
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The PCMT1 gene encodes the protein repair enzyme protein-L-isoaspartate (D-aspartate) O-methyltransferase, which is known to protect certain neural cells against Bax-induced apoptosis.

实验方案

Product Specific Protocols
WB protocol for PCMT1 antibody 60172-1-IgDownload protocol
IF protocol for PCMT1 antibody 60172-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols