验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
HRP-60172 targets PCMT1 in WB applications and shows reactivity with human, pig samples.
| 经测试应用 | WB Application Description |
| 经测试反应性 | human, pig |
| 免疫原 | PCMT1 fusion protein Ag6277 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | protein-L-isoaspartate (D-aspartate) O-methyltransferase |
| 别名 | PCMT1 |
| 计算分子量 | 25 kDa |
| 观测分子量 | 25-27 kDa |
| GenBank蛋白编号 | BC008748 |
| 基因名称 | PCMT1 |
| Gene ID (NCBI) | 5110 |
| RRID | AB_2920511 |
| 偶联类型 | HRP |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The PCMT1 gene encodes the protein repair enzyme protein-L-isoaspartate (D-aspartate) O-methyltransferase, which is known to protect certain neural cells against Bax-induced apoptosis.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HRP PCMT1 antibody HRP-60172 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |