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PCOLCE Polyclonal antibody

PCOLCE Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 28826-1-AP

Print datasheet

Synonyms

PCOLCE, PCPE, PCPE 1, PCPE1

验证数据展示

  • WB analysis using 28826-1-AP

    WB analysis using 28826-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28826-1-AP (PCOLCE antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inhuman plasma

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28826-1-AP targets PCOLCE in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human
Immunogen PCOLCE fusion protein Ag29719 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name procollagen C-endopeptidase enhancer
Synonyms PCOLCE, PCPE, PCPE 1, PCPE1
Calculated Molecular Weight 48 kDa
Observed Molecular Weight48-50 kDa
GenBank Accession NumberBC000574
Gene Symbol PCOLCE
Gene ID (NCBI) 5118
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15113
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PCOLCE (Procollagen C-endopeptidase enhancer 1), also named as PCPE1, is a secreted glycoprotein that functions as a positive regulator of procollagen processing. The calculated molecular weight of PCOLCE is 48 kDa. PCOLCE binds to the C-terminal propeptide of type I procollagen and enhances procollagen C-proteinase activity. Procollagen C-proteinases have important roles in developmental processes and assembly of the extracellular matrix. It has been reported that the dysregulation of PCOLCE is involved in numerous diseases. For instance, the expression of PCOLCE positively correlates with muscle and liver fibrosis (PMID: 27458976). And PCOLCE was highly expressed in osteosarcoma and may play an important role in promoting the lung metastasis of osteosarcoma (PMID: 31285765).

实验方案

Product Specific Protocols
WB protocol for PCOLCE antibody 28826-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols