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Host
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Species Reactivity
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Species Reactivity
0
Conjugate
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Conjugate
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Compatible Species
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Conjugate
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验证数据展示

  • WB analysis using 28826-1-AP

    WB analysis using 28826-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28826-1-AP (PCOLCE antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

PCOLCE Polyclonal antibody

PCOLCE Polyclonal Antibody for WB, ELISA
Cat No. 28826-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, ELISA

别名

PCOLCE, PCPE, PCPE 1, PCPE1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman plasma

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28826-1-AP targets PCOLCE in WB, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human
免疫原 PCOLCE fusion protein Ag29719 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 procollagen C-endopeptidase enhancer
别名 PCOLCE, PCPE, PCPE 1, PCPE1
计算分子量 48 kDa
观测分子量48-50 kDa
GenBank蛋白编号BC000574
基因名称 PCOLCE
Gene ID (NCBI) 5118
RRIDAB_3086087
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ15113
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PCOLCE (Procollagen C-endopeptidase enhancer 1), also named as PCPE1, is a secreted glycoprotein that functions as a positive regulator of procollagen processing. The calculated molecular weight of PCOLCE is 48 kDa. PCOLCE binds to the C-terminal propeptide of type I procollagen and enhances procollagen C-proteinase activity. Procollagen C-proteinases have important roles in developmental processes and assembly of the extracellular matrix. It has been reported that the dysregulation of PCOLCE is involved in numerous diseases. For instance, the expression of PCOLCE positively correlates with muscle and liver fibrosis (PMID: 27458976). And PCOLCE was highly expressed in osteosarcoma and may play an important role in promoting the lung metastasis of osteosarcoma (PMID: 31285765).

实验方案

Product Specific Protocols
WB protocol for PCOLCE antibody 28826-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols