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CoraLite® Plus 488-conjugated PCYT2 Monoclonal antibody

PCYT2 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2F7G1

Cat no : CL488-68355

Print datasheet

Synonyms

Ethanolamine-phosphate cytidylyltransferase, Ethanolamine phosphate cytidylyltransferase, EC:2.7.7.14, 2F7G1

验证数据展示

  • IF Staining of HepG2 using CL488-68355

    IF Staining of HepG2 using CL488-68355

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 PCYT2 antibody (CL488-68355, Clone: 2F7G1 ) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 68355-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68355 targets PCYT2 in IF/ICC applications and shows reactivity with human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human
Immunogen PCYT2 fusion protein Ag6604 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name phosphate cytidylyltransferase 2, ethanolamine
Synonyms Ethanolamine-phosphate cytidylyltransferase, Ethanolamine phosphate cytidylyltransferase, EC:2.7.7.14, 2F7G1
Calculated Molecular Weight 44 kDa
Observed Molecular Weight 44 kDa
GenBank Accession NumberBC000351
Gene Symbol PCYT2
Gene ID (NCBI) 5833
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ99447
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ethanolamine-phosphate cytidylyltransferase (PCYT2) catalyzes the second step in the synthesis of phosphatidylethanolamine (PE) from ethanolamine via the CDP-ethanolamine pathway (PMID:9083101, PMID:31637422). Phosphatidylethanolamine is a dominant inner-leaflet phospholipid in cell membranes, where it plays a role in membrane function by structurally stabilizing membrane-anchored proteins, and participates in important cellular processes such as cell division, cell fusion, blood coagulation, and apoptosis (PMID:9083101).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 PCYT2 antibody CL488-68355Download protocol
Standard Protocols
Click here to view our Standard Protocols