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PD-1/CD279 Monoclonal antibody

PD-1/CD279 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF-P, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

4H4D1

Cat no : 66220-1-Ig

Print datasheet

Synonyms

CD279, PD1, PD-1, 4H4D1, PD 1



经过测试的应用

Positive WB detected inRAW 264.7 cells, human lymph node tissue, rat spleen tissue, mouse thymus tissue, Jurkat cells, MOLT-4 cells, THP-1 cells, CTLL-2 cells, pig thymus tissue
Positive IHC detected inhuman tonsillitis tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue, human lymphoma tissue
Planning an IHC experiment? We recommend our IHCeasy PD-1 Ready-To-Use IHC Kit. PD-1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®594 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66220-1-Ig targets PD-1/CD279 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse, rat, pig
Cited Reactivityhuman, mouse, rat
Immunogen PD-1/CD279 fusion protein Ag12470 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name programmed cell death 1
Synonyms CD279, PD1, PD-1, 4H4D1, PD 1
Calculated Molecular Weight 288 aa, 32 kDa
Observed Molecular Weight 32 kDa, 47-55 kDa
GenBank Accession NumberBC074740
Gene Symbol PD-1
Gene ID (NCBI) 5133
RRIDAB_2881611
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ15116
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Programmed cell death 1 (PD-1, also known as CD279) is an immunoinhibitory receptor that belongs to the CD28/CTLA-4 subfamily of the Ig superfamily. It is a 288 amino acid (aa) type I transmembrane protein composed of one Ig superfamily domain, a stalk, a transmembrane domain, and an intracellular domain containing an immunoreceptor tyrosine-based inhibitory motif (ITIM) as well as an immunoreceptor tyrosine-based switch motif (ITSM) (PMID: 18173375). PD-1 is expressed during thymic development and is induced in a variety of hematopoietic cells in the periphery by antigen receptor signaling and cytokines (PMID: 20636820). Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function (PMID: 19426218). It is critical for the regulation of T cell function during immunity and tolerance. Blockade of PD-1 can overcome immune resistance and also has been shown to have antitumor activity (PMID: 22658127; 23169436). The calculated molecular weight of PD-1 is 32 kDa. It has been reported that PD-1 is heavily glycosylated and migrates with an apparent molecular mass of 47-55 kDa on SDS-PAGE (PMID: 8671665; 17640856; 17003438).

实验方案

Product Specific Protocols
WB protocol for PD-1/CD279 antibody 66220-1-IgDownload protocol
IHC protocol for PD-1/CD279 antibody 66220-1-IgDownload protocol
IF protocol for PD-1/CD279 antibody 66220-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseIHC,IF

Nat Commun

ERK and USP5 govern PD-1 homeostasis via deubiquitination to modulate tumor immunotherapy

Authors - Xiangling Xiao
humanWB

Nat Commun

m6A mRNA demethylase FTO regulates melanoma tumorigenicity and response to anti-PD-1 blockade.

Authors - Seungwon Yang
mouseWB

Cancers (Basel)

Overcoming PD-1 Inhibitor Resistance with a Monoclonal Antibody to Secreted Frizzled-Related Protein 2 in Metastatic Osteosarcoma.

Authors - Patrick Nasarre
human,mouseWB,IHC

Biochem Pharmacol

Targeting HMGA1 contributes to immunotherapy in aggressive breast cancer while suppressing EMT

Authors - Xing Chang
human,mouseWB,IF

Int Immunopharmacol

EGCG suppresses PD-1 expression of T cells via inhibiting NF-κB phosphorylation and nuclear translocation

Authors - Zhong-Da Li
mouseWB

Int Immunopharmacol

HMGB1 promotes neutrophil PD-L1 expression through TLR2 and mediates T cell apoptosis leading to immunosuppression in sepsis

Authors - Jinlian Liu