- Featured Product
- KD/KO Validated
PD-1/CD279 Monoclonal antibody
PD-1/CD279 Monoclonal Antibody for WB, IHC, IF-P, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human, mouse, rat, pig
Applications
WB, IHC, IF-P, ELISA and More (1)
Conjugate
Unconjugated
CloneNo.
4H4D1
验证数据展示
经过测试的应用
Positive WB detected in | RAW 264.7 cells, human lymph node tissue, rat spleen tissue, mouse thymus tissue, Jurkat cells, MOLT-4 cells, THP-1 cells, CTLL-2 cells, pig thymus tissue |
Positive IHC detected in | human tonsillitis tissue, human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | human tonsillitis tissue, human lymphoma tissue |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
66220-1-Ig targets PD-1/CD279 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
Tested Applications | WB, IHC, IF-P, ELISA Application Description |
Cited Applications | WB, IHC, IF |
Tested Reactivity | human, mouse, rat, pig |
Cited Reactivity | human, mouse, rat |
Immunogen | PD-1/CD279 fusion protein Ag12470 种属同源性预测 |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Full Name | programmed cell death 1 |
Synonyms | CD279, PD1, PD-1, 4H4D1, PD 1 |
Calculated Molecular Weight | 288 aa, 32 kDa |
Observed Molecular Weight | 32 kDa, 47-55 kDa |
GenBank Accession Number | BC074740 |
Gene Symbol | PD-1 |
Gene ID (NCBI) | 5133 |
RRID | AB_2881611 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q15116 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for PD-1/CD279 antibody 66220-1-Ig | Download protocol |
IHC protocol for PD-1/CD279 antibody 66220-1-Ig | Download protocol |
IF protocol for PD-1/CD279 antibody 66220-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Commun ERK and USP5 govern PD-1 homeostasis via deubiquitination to modulate tumor immunotherapy | ||
Nat Commun m6A mRNA demethylase FTO regulates melanoma tumorigenicity and response to anti-PD-1 blockade. | ||
Cancers (Basel) Overcoming PD-1 Inhibitor Resistance with a Monoclonal Antibody to Secreted Frizzled-Related Protein 2 in Metastatic Osteosarcoma. | ||
Biochem Pharmacol Targeting HMGA1 contributes to immunotherapy in aggressive breast cancer while suppressing EMT | ||
Int Immunopharmacol EGCG suppresses PD-1 expression of T cells via inhibiting NF-κB phosphorylation and nuclear translocation | ||
Int Immunopharmacol HMGB1 promotes neutrophil PD-L1 expression through TLR2 and mediates T cell apoptosis leading to immunosuppression in sepsis |