- Featured Product
- KD/KO Validated
CoraLite® Plus 647-conjugated PD-1/CD279 Monoclonal antibody
PD-1/CD279 Monoclonal Antibody for IF-P, FC
Host / Isotype
Mouse / IgG2b
Reactivity
human, mouse, rat
Applications
IF-P, FC and More (1)
Conjugate
CoraLite® Plus 647 Fluorescent Dye
CloneNo.
4H4D1
验证数据展示
经过测试的应用
Positive IF-P detected in | human tonsillitis tissue |
Positive FC detected in | human PBMCs, PBMC |
推荐稀释比
Application | Dilution |
---|---|
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
IF | See 1 publications below |
FC | See 1 publications below |
产品信息
CL647-66220 targets PD-1/CD279 in IF-P, FC applications and shows reactivity with human, mouse, rat samples.
Tested Applications | IF-P, FC Application Description |
Cited Applications | IF, FC |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | mouse, rat |
Immunogen | PD-1/CD279 fusion protein Ag12470 种属同源性预测 |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Full Name | programmed cell death 1 |
Synonyms | CD279, PD1, PD-1, 4H4D1, PD 1 |
Calculated Molecular Weight | 288 aa, 32 kDa |
GenBank Accession Number | BC074740 |
Gene Symbol | PD-1 |
Gene ID (NCBI) | 5133 |
RRID | AB_2883732 |
Conjugate | CoraLite® Plus 647 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 654 nm / 674 nm |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q15116 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 647 PD-1/CD279 antibody CL647-66220 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Clin Cancer Res 18F-FMISO PET Imaging Identifies Hypoxia and Immunosuppressive Tumor Microenvironments and Guides Targeted Evofosfamide Therapy in Tumors Refractory to PD-1 and CTLA-4 Inhibition. | ||
Transplantation Activation of the Aryl Hydrocarbon Receptor Ameliorates Acute Rejection of Rat Liver Transplantation by Regulating Treg Proliferation and PD-1 Expression. |