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CoraLite® Plus 647-conjugated PD-1/CD279 Monoclonal antibody

PD-1/CD279 Monoclonal Antibody for IF-P, FC

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

IF-P, FC and More (1)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

4H4D1

Cat no : CL647-66220

Print datasheet

Synonyms

CD279, PD1, PD-1, 4H4D1, PD 1



经过测试的应用

Positive IF-P detected inhuman tonsillitis tissue
Positive FC detected inhuman PBMCs, PBMC
Planning an IHC experiment? We recommend our IHCeasy PD-1 Ready-To-Use IHC Kit. PD-1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66220-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-66220 targets PD-1/CD279 in IF-P, FC applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF-P, FC Application Description
Cited ApplicationsIF, FC
Tested Reactivity human, mouse, rat
Cited Reactivitymouse, rat
Immunogen PD-1/CD279 fusion protein Ag12470 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name programmed cell death 1
Synonyms CD279, PD1, PD-1, 4H4D1, PD 1
Calculated Molecular Weight 288 aa, 32 kDa
GenBank Accession NumberBC074740
Gene Symbol PD-1
Gene ID (NCBI) 5133
RRIDAB_2883732
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ15116
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Programmed cell death 1 (PD-1, also known as CD279) is an immunoinhibitory receptor that belongs to the CD28/CTLA-4 subfamily of the Ig superfamily. It is a 288 amino acid (aa) type I transmembrane protein composed of one Ig superfamily domain, a stalk, a transmembrane domain, and an intracellular domain containing an immunoreceptor tyrosine-based inhibitory motif (ITIM) as well as an immunoreceptor tyrosine-based switch motif (ITSM) (PMID: 18173375). PD-1 is expressed during thymic development and is induced in a variety of hematopoietic cells in the periphery by antigen receptor signaling and cytokines (PMID: 20636820). Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function (PMID: 19426218). It is critical for the regulation of T cell function during immunity and tolerance. Blockade of PD-1 can overcome immune resistance and also has been shown to have antitumor activity (PMID: 22658127; 23169436). The calculated molecular weight of PD-1 is 32 kDa. It has been reported that PD-1 is heavily glycosylated and migrates with an apparent molecular mass of 47-55 kDa on SDS-PAGE (PMID: 8671665; 17640856; 17003438).

实验方案

Product Specific Protocols
IF protocol for CL Plus 647 PD-1/CD279 antibody CL647-66220Download protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Clin Cancer Res

18F-FMISO PET Imaging Identifies Hypoxia and Immunosuppressive Tumor Microenvironments and Guides Targeted Evofosfamide Therapy in Tumors Refractory to PD-1 and CTLA-4 Inhibition.

Authors - Kirsten M Reeves
ratFC

Transplantation

Activation of the Aryl Hydrocarbon Receptor Ameliorates Acute Rejection of Rat Liver Transplantation by Regulating Treg Proliferation and PD-1 Expression.

Authors - Wanyue Cao