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Anti-Human PD-1/CD279 Rabbit Recombinant Antibody, PBS Only

PD-1/CD279 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

240724G11

Cat no : 98068-1-PBS

Print datasheet

Synonyms

CD279, PD1, PD-1, 240724G11, PD 1

验证数据展示

  • FC experiment of human PBMCs using 98068-1-RR (same clone as 98068-1-PBS)

    FC experiment of human PBMCs using 98068-1-RR (same clone as 98068-1-PBS)

    1x10^6 unstimulated or PHA stimulated human PBMCs were stained with 0.25 ug Anti-Human PD-1/CD279 Rabbit Recombinant Antibody (98068-1-RR, Clone:240724G11) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with APC Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (APC-65569, Clone: OKT3). Cells were not fixed. This data was developed using the same antibody clone with 98068-1-PBS in a different storage buffer formulation.

  • FC experiment of human PBMCs using 98068-1-RR (same clone as 98068-1-PBS)

    FC experiment of human PBMCs using 98068-1-RR (same clone as 98068-1-PBS)

    1x10^6 PHA stimulated human PBMCs were stained with 0.25 ug Anti-Human PD-1/CD279 Rabbit Recombinant Antibody (98068-1-RR, Clone:240724G11)(left) or 0.25 ug PE Anti-Human PD-1/CD279 (J110) (PE-65119, Clone: J110)(right) and PE-Conjugated Goat Anti-Rabbit IgG(H+L), Cells were then stained with APC Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (APC-65569, Clone: OKT3). Cells were not fixed. This data was developed using the same antibody clone with 98068-1-PBS in a different storage buffer formulation.

  • FC experiment of human PBMCs using 98068-1-RR (same clone as 98068-1-PBS)

    FC experiment of human PBMCs using 98068-1-RR (same clone as 98068-1-PBS)

    1x10^6 unstimulated (black) or PHA stimulated human PBMCs (red) were stained with 0.25 ug Anti-Human PD-1/CD279 Rabbit Recombinant Antibody (98068-1-RR, Clone:240724G11) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 PHA treated human PBMCs were stained with Isotype Control(blue). Cells were not fixed. This data was developed using the same antibody clone with 98068-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 98068-1-RR

    Affinity and Kinetic Characterization of 98068-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98068-1-RR against Human PD-1/CD279 were performed. The affinity constant is 0.966 nM.

Planning an IHC experiment? We recommend our IHCeasy PD-1 Ready-To-Use IHC Kit. PD-1 primary antibody included.

产品信息

98068-1-PBS targets PD-1/CD279 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name programmed cell death 1
Synonyms CD279, PD1, PD-1, 240724G11, PD 1
Calculated Molecular Weight 288 aa, 32 kDa
GenBank Accession NumberBC074740
Gene Symbol PD-1
Gene ID (NCBI) 5133
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ15116
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Programmed cell death 1 (PD-1, also known as CD279) is an immunoinhibitory receptor that belongs to the CD28/CTLA-4 subfamily of the Ig superfamily. It is a 288 amino acid (aa) type I transmembrane protein composed of one Ig superfamily domain, a stalk, a transmembrane domain, and an intracellular domain containing an immunoreceptor tyrosine-based inhibitory motif (ITIM) as well as an immunoreceptor tyrosine-based switch motif (ITSM) (PMID: 18173375). PD-1 is expressed during thymic development and is induced in a variety of hematopoietic cells in the periphery by antigen receptor signaling and cytokines (PMID: 20636820). Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function (PMID: 19426218). It is critical for the regulation of T cell function during immunity and tolerance. Blockade of PD-1 can overcome immune resistance and also has been shown to have antitumor activity (PMID: 22658127; 23169436). It has been reported that PD-1 is heavily glycosylated and migrates with an apparent molecular mass of 47-55 kDa on SDS-PAGE , which is larger than its predicted mass of 32 kDa (PMID: 8671665; 17640856; 17003438).